Mohsin S N, Ghafoor F, Saleem M, Ghous R, Aasim M
Pakistan Medical Research Council,National Health Research Complex,Shaikh Zayed Medical Complex,Lahore,Pakistan.
Institute of Biochemistry and Biotechnology,University of Punjab,Lahore,Pakistan.
Epidemiol Infect. 2016 Aug;144(11):2276-82. doi: 10.1017/S0950268816000522. Epub 2016 Mar 28.
Dengue has become a global problem in past few decades, with half of the world's population at risk of infection. For some countries of Asia and Latin America, severe dengue is a major cause of serious illness and even death in children. Pakistan has been reported as a hyperendemic area for dengue infection. Our study aimed to find seroprevalence of past dengue infection in asymptomatic children of Lahore with no previous history of dengue infection. A total of 400 samples were collected from children aged 1-12 years in Lahore using random sampling. The inclusion criteria were children aged 1-12 years, who had no previous symptoms of dengue fever during their lives. Children with known immunodeficiency status or fever at the time of recruitment were excluded from the study. Commercially available ELISA kits were used to determine the IgG status in sera of children. The data obtained was entered and analysed using SPSS v. 20.0. The overall prevalence of asymptomatic dengue infection was found to be 25%. There was no statistically significant difference between prevalence of infection in male and female children. There was, however, a strong relationship between increasing age of the child and number of cases with infection, with low incidence in children aged ⩽5 years.
在过去几十年里，登革热已成为一个全球性问题，全球一半人口面临感染风险。对于亚洲和拉丁美洲的一些国家而言，严重登革热是儿童重病甚至死亡的主要原因。据报道，巴基斯坦是登革热感染的高度流行地区。我们的研究旨在找出拉合尔市无登革热感染既往史的无症状儿童中既往登革热感染的血清阳性率。采用随机抽样的方法，从拉合尔市1至12岁的儿童中总共采集了400份样本。纳入标准为年龄在1至12岁、一生中无登革热发热既往症状的儿童。已知免疫缺陷状态或在招募时发热的儿童被排除在研究之外。使用市售酶联免疫吸附测定（ELISA）试剂盒测定儿童血清中的IgG状态。将获得的数据录入并使用SPSS v. 20.0进行分析。发现无症状登革热感染的总体患病率为25%。男童和女童的感染患病率之间无统计学显著差异。然而，儿童年龄增长与感染病例数之间存在密切关系，5岁及以下儿童的发病率较低。
