School of Public Health, Sun Yat-sen University, Guangzhou 510080, China.
Department of Infectious Disease, Guangzhou Centre for Disease Control and Prevention, Guangzhou 510440, China.
Int J Environ Res Public Health. 2018 Jun 11;15(6):1227. doi: 10.3390/ijerph15061227.
The majority of dengue virus infections are asymptomatic, which could potentially facilitate the transmission of dengue fever and increase the percentage of sever dengue fever manifestations. This cross-sectional study explored the sero-prevalence of dengue virus infection in Guangzhou to clarify the infection spectrum. In total, 2085 serum samples were collected from residents of 34 communities. All samples were selected from a 200,000-sample database holding serum collected from community residents living in Liwan and Yuexiu districts of Guangzhou between September 2013 and August 2015, and 17 to 28 individuals of each age group were chosen per month. Dengue immunoglobulin G (IgG) and immunoglobulin M (IgM) antibodies were tested by enzyme-linked immunosorbent assay. Symptomatic infected individuals were identified via follow-up questionnaires. Among 2085 serum samples, anti-dengue IgG and IgM positive rates were 11.80% and 3.98%, respectively. The IgG antibody positive rate increased with age and was higher in poorly educated people than in highly educated people and in married individuals than in single individuals. Approximately 96.71% of dengue virus infections and an estimated 13.68% of the whole population were asymptomatic. Such high asymptomatic-infection rates have an impact on the local spread of dengue fever. Stricter surveillance, such as a network of rapid diagnostic laboratories, screening of residents in the epidemic season, and other integrated control measures are necessary.
大多数登革热病毒感染为无症状感染，这可能会促进登革热的传播，并增加重症登革热表现的比例。本横断面研究旨在探索广州登革病毒感染的血清流行率，以阐明感染谱。共采集了 34 个社区的 2085 份血清样本。所有样本均选自 2013 年 9 月至 2015 年 8 月期间采集的居住在广州荔湾区和越秀区的社区居民的血清数据库，每个月选择每个年龄组的 17 至 28 个人。采用酶联免疫吸附试验检测登革病毒免疫球蛋白 G（IgG）和免疫球蛋白 M（IgM）抗体。通过随访问卷确定有症状的感染个体。在 2085 份血清样本中，抗登革 IgG 和 IgM 阳性率分别为 11.80%和 3.98%。IgG 抗体阳性率随年龄增长而增加，且受教育程度较低者高于受教育程度较高者，已婚者高于单身者。约 96.71%的登革病毒感染和估计 13.68%的整个人群为无症状感染。如此高的无症状感染率对当地登革热的传播有影响。有必要加强监测，如建立快速诊断实验室网络、在流行季节对居民进行筛查，以及采取其他综合控制措施。
