Jarukamjorn Kanokwan, Jearapong Nattharat, Pimson Charinya, Chatuphonprasert Waranya
Research Group for Pharmaceutical Activities of Natural Products Using Pharmaceutical Biotechnology (PANPB), Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
Faculty of Medicine, Mahasarakham University, Mahasarakham 44000, Thailand.
Scientifica (Cairo). 2016;2016:5029414. doi: 10.1155/2016/5029414. Epub 2016 Feb 25.
Excessive fat liver is an important manifestation of nonalcoholic fatty liver disease (NAFLD), associated with obesity, insulin resistance, and oxidative stress. In the present study, the effects of a high-fat, high-fructose diet (HFFD) on mRNA levels and activities of the antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), were determined in mouse livers and brains. The histomorphology of the livers was examined and the state of nonenzymatic reducing system was evaluated by measuring the glutathione system and the lipid peroxidation. Histopathology of the liver showed that fat accumulation and inflammation depended on the period of the HFFD-consumption. The levels of mRNA and enzymatic activities of SOD, CAT, and GPx were raised, followed by the increases in malondialdehyde levels in livers and brains of the HFFD mice. The oxidized GSSG content was increased while the total GSH and the reduced GSH were decreased, resulting in the increase in the GSH/GSSG ratio in both livers and brains of the HFFD mice. These observations suggested that liver damage and oxidative stress in the significant organs were generated by continuous HFFD-consumption. Imbalance of antioxidant condition induced by long-term HFFD-consumption might increase the risk and progression of NAFLD.
脂肪肝是非酒精性脂肪性肝病（NAFLD）的重要表现，与肥胖、胰岛素抵抗和氧化应激相关。在本研究中，测定了高脂高果糖饮食（HFFD）对小鼠肝脏和大脑中抗氧化酶（包括超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx））的mRNA水平和活性的影响。检查了肝脏的组织形态学，并通过测量谷胱甘肽系统和脂质过氧化来评估非酶还原系统的状态。肝脏组织病理学显示，脂肪堆积和炎症取决于HFFD摄入的时间。HFFD小鼠肝脏和大脑中SOD、CAT和GPx的mRNA水平和酶活性升高，随后丙二醛水平增加。HFFD小鼠肝脏和大脑中氧化型谷胱甘肽（GSSG）含量增加，而总谷胱甘肽（GSH）和还原型谷胱甘肽（GSH）减少，导致GSH/GSSG比值增加。这些观察结果表明，持续摄入HFFD会导致重要器官的肝损伤和氧化应激。长期摄入HFFD引起的抗氧化状态失衡可能会增加NAFLD的风险和进展。
