Stepanova E O, Nikolaeva M A, Golubeva E L, Vtorushina V V, Van'ko L V, Khodzhaeva Z S, Krechetova L V
V. I. Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
Bull Exp Biol Med. 2016 Mar;160(5):722-6. doi: 10.1007/s10517-016-3260-2. Epub 2016 Mar 29.
Anti-HLA-antibodies were detected using cross-reaction of blood serum with allogenic T and B cells and latex microspheres coated with HLA-I and HLA-II antigens. HLA+ and HLA-sera obtained from women before and after allogeneic immunization were tested. The results obtained by these methods significantly differed. The test with latex microspheres detected antibodies to HLA-I and HLA-II antigens with high sensitivity and specificity and can be used for assessment of clinical significance of alloantibody detection when using alloimmunization in the therapy of gestation disorders.
通过血清与同种异体T细胞和B细胞以及包被有HLA - I和HLA - II抗原的乳胶微球的交叉反应来检测抗HLA抗体。对同种异体免疫前后女性获得的HLA +和HLA -血清进行检测。这些方法获得的结果有显著差异。乳胶微球检测对HLA - I和HLA - II抗原的抗体具有高灵敏度和特异性，可用于评估在妊娠疾病治疗中使用同种异体免疫时同种抗体检测的临床意义。
