磁场有助于细胞摄取，从而实现使用编码针对 Mcam 的质粒 DNA 的磁转染在体内对 B16F10 黑色素瘤的有效治疗。
Magnetic field contributes to the cellular uptake for effective therapy with magnetofection using plasmid DNA encoding against Mcam in B16F10 melanoma in vivo.
机构信息
Department of Experimental Oncology, Institute of Oncology Ljubljana, Zaloska 2, SI-1000 Ljubljana, Slovenia.
Institute of Cell Biology, Faculty of Medicine, University of Ljubljana, Vrazov trg 2, SI-1000 Ljubljana, Slovenia.
出版信息
Nanomedicine (Lond). 2016 Mar;11(6):627-41. doi: 10.2217/nnm.16.4. Epub 2016 Mar 8.
AIM
We explored the distribution and cellular uptake of intratumorally injected SPIONs-PAA-PEI-pDNA (magnetofection complexes), and antitumor effectiveness of magnetofection with plasmid DNA encoding short hairpin RNA (shRNA) against Mcam (pDNA(anti-MCAM)).
MATERIALS & METHODS: Analyses were made based on the histology, ultrastructure and quantitative measurements of magnetofection complexes, and quantification of the antitumor effectiveness in B16F10 melanoma in vivo.
RESULTS
Injected magnetofection complexes were distributed around the injection site. Exposure of tumors to external magnetic field contributed to the uptake of magnetofection complexes from extracellular matrix into melanoma cells. Three consecutive magnetofections of tumors with pDNA(anti-MCAM) resulted in significant reduction of tumor volume.
CONCLUSION
Magnetofection is effective for gene delivery to melanoma tumors, but requires a magnetic field for cellular uptake and antitumor effect.
目的
我们研究了肿瘤内注射 SPIONs-PAA-PEI-pDNA（磁转染复合物）的分布和细胞摄取情况，以及用编码短发夹 RNA（shRNA）针对 Mcam 的质粒 DNA（pDNA(anti-MCAM)）进行磁转染的抗肿瘤效果。
材料与方法
分析基于磁转染复合物的组织学、超微结构和定量测量，以及体内 B16F10 黑色素瘤的抗肿瘤效果的定量。
结果
注射的磁转染复合物分布在注射部位周围。肿瘤对外磁场的暴露有助于磁转染复合物从细胞外基质进入黑色素瘤细胞。对肿瘤进行三次连续的 pDNA(anti-MCAM)磁转染导致肿瘤体积显著减小。
结论
磁转染可有效将基因递送至黑色素瘤肿瘤，但需要磁场以实现细胞摄取和抗肿瘤效果。
Zotero 插件