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携带绿色荧光蛋白编码DNA的聚乙烯亚胺包被的超顺磁性氧化铁纳米颗粒对人乳腺癌细胞的磁转染

Magnetofection of Green Fluorescent Protein Encoding DNA-Bearing Polyethyleneimine-Coated Superparamagnetic Iron Oxide Nanoparticles to Human Breast Cancer Cells.

作者信息

Zuvin Merve, Kuruoglu Efe, Kaya Veysel Ogulcan, Unal Ozlem, Kutlu Ozlem, Yagci Acar Havva, Gozuacik Devrim, Koşar Ali

机构信息

Mechatronics Engineering Program, Faculty of Engineering and Natural Sciences, Molecular Biology, Genetics and Bioengineering Program, Faculty of Engineering and Natural Sciences, and Center of Excellence for Functional Surfaces and Interfaces for Nano Diagnostics (EFSUN), Sabanci University, Orhanli, 34956 Tuzla, Istanbul, Turkey.

Department of Chemistry, Faculty of Arts and Sciences, Koc University, 34450 Sariyer, Istanbul, Turkey.

出版信息

ACS Omega. 2019 Jul 18;4(7):12366-12374. doi: 10.1021/acsomega.9b01000. eCollection 2019 Jul 31.

DOI:10.1021/acsomega.9b01000
PMID:31460354
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6682024/
Abstract

Gene therapy is a developing method for the treatment of various diseases. For this purpose, the search for nonviral methods has recently accelerated to avoid toxic effects. A strong alternative method is magnetofection, which involves the use of superparamagnetic iron oxide nanoparticles (SPIONs) with a proper organic coating and external magnetic field to enhance the localization of SPIONs at the target site. In this study, a new magnetic actuation system consisting of four rare-earth magnets on a rotary table was designed and manufactured to obtain improved magnetofection. As a model, green fluorescent protein DNA-bearing polyethyleneimine-coated SPIONs were used. Magnetofection was tested on MCF7 cells. The system reduced the transfection time (down to 1 h) of the standard polyethyleneimine transfection protocol. As a result, we showed that the system could be effectively used for gene transfer.

摘要

基因治疗是一种用于治疗各种疾病的新兴方法。为此，最近加速了对非病毒方法的探索，以避免毒性作用。一种强有力的替代方法是磁转染，它涉及使用具有适当有机涂层的超顺磁性氧化铁纳米颗粒（SPIONs）和外部磁场，以增强SPIONs在靶位点的定位。在本研究中，设计并制造了一种由旋转台上的四个稀土磁体组成的新型磁驱动系统，以实现改进的磁转染。作为模型，使用了携带绿色荧光蛋白DNA的聚乙烯亚胺包被的SPIONs。在MCF7细胞上测试了磁转染。该系统缩短了标准聚乙烯亚胺转染方案的转染时间（缩短至1小时）。结果表明，该系统可有效地用于基因转移。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ed9/6682024/86b010d02c96/ao-2019-010002_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ed9/6682024/dff33d641ee3/ao-2019-010002_0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ed9/6682024/0ba463f9bdf8/ao-2019-010002_0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ed9/6682024/7ab218683c18/ao-2019-010002_0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ed9/6682024/37b528ddce42/ao-2019-010002_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ed9/6682024/9f6b61017edf/ao-2019-010002_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ed9/6682024/3edcdcd0e579/ao-2019-010002_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ed9/6682024/5510dba945b5/ao-2019-010002_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ed9/6682024/86b010d02c96/ao-2019-010002_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ed9/6682024/dff33d641ee3/ao-2019-010002_0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ed9/6682024/0ba463f9bdf8/ao-2019-010002_0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ed9/6682024/7ab218683c18/ao-2019-010002_0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ed9/6682024/37b528ddce42/ao-2019-010002_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ed9/6682024/9f6b61017edf/ao-2019-010002_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ed9/6682024/3edcdcd0e579/ao-2019-010002_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ed9/6682024/5510dba945b5/ao-2019-010002_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ed9/6682024/86b010d02c96/ao-2019-010002_0003.jpg

相似文献

[1]
Magnetofection of Green Fluorescent Protein Encoding DNA-Bearing Polyethyleneimine-Coated Superparamagnetic Iron Oxide Nanoparticles to Human Breast Cancer Cells.

ACS Omega. 2019-7-18

[2]
Effect of Varying Magnetic Fields on Targeted Gene Delivery of Nucleic Acid-Based Molecules.

Ann Biomed Eng. 2015-11

[3]
Plasmid-DNA Delivery by Covalently Functionalized PEI-SPIONs as a Potential 'Magnetofection' Agent.

Molecules. 2022-11-1

[4]
Polyethyleneimine-associated polycaprolactone-Superparamagnetic iron oxide nanoparticles as a gene delivery vector.

J Biomed Mater Res B Appl Biomater. 2017-1

[5]
Chondroitin sulfate-polyethylenimine copolymer-coated superparamagnetic iron oxide nanoparticles as an efficient magneto-gene carrier for microRNA-encoding plasmid DNA delivery.

Nanoscale. 2015-5-14

[6]
Increased cellular uptake of biocompatible superparamagnetic iron oxide nanoparticles into malignant cells by an external magnetic field.

J Membr Biol. 2010-7-3

[7]
Magnetofection: a reproducible method for gene delivery to melanoma cells.

Biomed Res Int. 2013-6-3

[8]
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Langmuir. 2011-3-1

[9]
Surface modified magnetic nanoparticles for immuno-gene therapy of murine mammary adenocarcinoma.

Biomaterials. 2012-3-18

[10]
Superparamagnetic nanoparticle delivery of DNA vaccine.

Methods Mol Biol. 2014

引用本文的文献

[1]
Effective gene delivery using size dependant nano core-shell in human cervical cancer cell lines by magnetofection.

PLoS One. 2023

[2]
Heterologous Expression of Codon-Optimized Azurin Transferred by Magnetofection Method in MCF-10A Cells.

Mol Biotechnol. 2024-6

[3]
Plant biomacromolecule delivery methods in the 21st century.

Front Genome Ed. 2022-10-14

[4]
Magnetofection approach for the transformation of okra using green iron nanoparticles.

Sci Rep. 2022-10-4

[5]
Magnetite Nanoparticles in Magnetic Hyperthermia and Cancer Therapies: Challenges and Perspectives.

Nanomaterials (Basel). 2022-5-25

[6]
Iron oxide nanoparticles as multimodal imaging tools.

RSC Adv. 2019-12-6

[7]
Magnetite Nanoparticles: Synthesis and Applications in Optics and Nanophotonics.

Materials (Basel). 2022-4-1

[8]
Aspects of high-performance and bio-acceptable magnetic nanoparticles for biomedical application.

Asian J Pharm Sci. 2021-11

[9]
Advances in Magnetic Nanoparticles Engineering for Biomedical Applications-A Review.

Bioengineering (Basel). 2021-9-30

[10]
A review of the tortuous path of nonviral gene delivery and recent progress.

Int J Biol Macromol. 2021-7-31

本文引用的文献

[1]
Assembly of polyethylenimine-functionalized iron oxide nanoparticles as agents for DNA transfection with magnetofection technique.

J Mater Chem B. 2014-12-7

[2]
Improving Magnetofection of Magnetic Polyethylenimine Nanoparticles into MG-63 Osteoblasts Using a Novel Uniform Magnetic Field.

Nanoscale Res Lett. 2019-3-12

[3]
Tumor angiogenesis and anti-angiogenic gene therapy for cancer.

Oncol Lett. 2018-7

[4]
Oncolytic Viral Therapy and the Immune System: A Double-Edged Sword Against Cancer.

Front Immunol. 2018-4-26

[5]
Glioblastoma Targeted Gene Therapy Based on pEGFP/p53-Loaded Superparamagnetic Iron Oxide Nanoparticles.

Curr Gene Ther. 2017

[6]
Oscillating Magnet Array-Based Nanomagnetic Gene Transfection: A Valuable Tool for Molecular Neurobiology Studies.

Nanomaterials (Basel). 2017-1-29

[7]
Development of an optimized AAV2/5 gene therapy vector for Leber congenital amaurosis owing to defects in RPE65.

Gene Ther. 2016-12

[8]
Fast, Efficient, and Gentle Transfection of Human Adherent Cells in Suspension.

ACS Appl Mater Interfaces. 2016-4-1

[9]
Magnetic field contributes to the cellular uptake for effective therapy with magnetofection using plasmid DNA encoding against Mcam in B16F10 melanoma in vivo.

Nanomedicine (Lond). 2016-3-8

[10]
Approach to Rapid Synthesis and Functionalization of Iron Oxide Nanoparticles for High Gene Transfection.

ACS Appl Mater Interfaces. 2016-3

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