Department of Dermatology, Kyoto University Graduate School of Medicine, Kyoto, Japan.
Department of Dermatology, Kyoto University Graduate School of Medicine, Kyoto, Japan.
Allergol Int. 2016 Jul;65(3):228-34. doi: 10.1016/j.alit.2016.02.008. Epub 2016 Mar 26.
In vivo imaging is a novel experimental approach for biological research. Multiphoton microscopy (MPM), a type of fluorescence microscopy, is a new tool for in vivo imaging analysis. MPM allows observation of both tissue structures and cell behaviors or cell-cell interactions in living animals in real time. Skin is an ideal tissue for MPM analysis as it is directly accessible to the microscope. In the skin, immune cells cooperate to maintain skin homeostasis or to exert immune responses against foreign antigens. In vivo imaging by MPM analysis provides precise information on cell dynamics in the skin, and has significantly expanded our knowledge of the cutaneous immune system. In this review, we will discuss recent insights related to the mechanisms of allergic skin inflammation that have been revealed by MPM analysis.
在体成像是一种用于生物研究的新型实验方法。多光子显微镜（MPM）是一种荧光显微镜，是一种用于活体成像分析的新工具。MPM 允许实时观察活体动物的组织结构和细胞行为或细胞-细胞相互作用。皮肤是 MPM 分析的理想组织，因为它可以直接接触显微镜。在皮肤中，免疫细胞合作以维持皮肤的稳态或对异物抗原产生免疫反应。通过 MPM 分析的活体成像提供了皮肤中细胞动力学的精确信息，并极大地扩展了我们对皮肤免疫系统的认识。在这篇综述中，我们将讨论 MPM 分析揭示的与过敏皮肤炎症机制相关的最新见解。
