Schießl Ina Maria, Castrop Hayo
Institute of Physiology, University of Regensburg, Universitätsstr. 31, 93040, Regensburg, Germany.
Pflugers Arch. 2016 Sep;468(9):1505-16. doi: 10.1007/s00424-016-1832-7. Epub 2016 Jun 28.
Intravital multiphoton microscopy is widely used to assess the structure and function of organs in live animals. Although different tissues vary in their accessibility for intravital multiphoton imaging, considerable progress has been made in the imaging quality of all tissues due to substantial technical improvements in the relevant imaging components, such as optics, excitation laser, detectors, and signal analysis software. In this review, we provide an overview of the technical background of intravital multiphoton microscopy. Then, we note a few seminal findings that were made through the use of multiphoton microscopy. Finally, we address the technical limitations of the method and provide an outlook for how these limitations may be overcome through future technical developments.
活体多光子显微镜广泛用于评估活体动物器官的结构和功能。尽管不同组织在活体多光子成像中的可及性有所不同，但由于光学、激发激光、探测器和信号分析软件等相关成像组件的重大技术改进，所有组织的成像质量都取得了显著进展。在本综述中，我们概述了活体多光子显微镜的技术背景。然后，我们列举了一些通过使用多光子显微镜取得的开创性发现。最后，我们讨论了该方法的技术局限性，并对如何通过未来的技术发展克服这些局限性进行了展望。
