Leveque Ryan E, Clark Karl J, Ekker Stephen C
Biochemistry and Molecular Biology, Mayo Clinic , Rochester, Minnesota.
Zebrafish. 2016 Jul;13 Suppl 1(Suppl 1):S44-6. doi: 10.1089/zeb.2015.1227. Epub 2016 Mar 29.
The zebrafish (Danio rerio) is a premier nonmammalian vertebrate model organism. This small aquatic fish is utilized in multiple disciplines in the Mayo Clinic community and by many laboratories around the world because of its biological similarity to humans, its advanced molecular genetics, the elucidation of its genome sequence, and the ever-expanding and outstanding new biological tools now available to the zebrafish researcher. The Mayo Clinic Zebrafish Facility (MCZF) houses ∼2,000 tanks annotated using an in-house, Internet cloud-based bar-coding system tied to our established zfishbook.org web infrastructure. Paramecia are the primary food source for larval fish rearing, using a simplified culture protocol described herein. The MCZF supports the specific ongoing research in a variety of laboratories, while also serving as a local hub for new scientists as they learn to tap into the potential of this model system for understanding normal development, disease, and as models of health.
斑马鱼（Danio rerio）是一种重要的非哺乳动物脊椎动物模型生物。这种小型水生鱼类因其与人类的生物学相似性、先进的分子遗传学、基因组序列的阐明以及斑马鱼研究人员现在可用的不断扩展且出色的新生物学工具，而被梅奥诊所社区的多个学科以及世界各地的许多实验室所使用。梅奥诊所斑马鱼设施（MCZF）容纳了约2000个水箱，这些水箱使用与我们已建立的zfishbook.org网络基础设施相关联的内部基于互联网云的条形码系统进行标注。草履虫是幼鱼饲养的主要食物来源，采用本文所述的简化培养方案。MCZF支持各个实验室正在进行的特定研究，同时也作为新科学家的本地中心，帮助他们学习挖掘这个模型系统在理解正常发育、疾病以及健康模型方面的潜力。
