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斑马鱼数据库:一种用于水生模式生物的直观追踪解决方案。

Zebrabase: An Intuitive Tracking Solution for Aquatic Model Organisms.

作者信息

Oltova Jana, Jindrich Jindrich, Skuta Ctibor, Svoboda Ondrej, Machonova Olga, Bartunek Petr

机构信息

1 Department of Cell Differentiation, Institute of Molecular Genetics AS CR v.v.i. , Prague, Czech Republic .

2 CZ-OPENSCREEN, Institute of Molecular Genetics AS CR v.v.i. , Prague, Czech Republic .

出版信息

Zebrafish. 2018 Dec;15(6):642-647. doi: 10.1089/zeb.2018.1609. Epub 2018 Sep 20.

DOI:10.1089/zeb.2018.1609
PMID:30234459
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6277078/
Abstract

Small fish species, such as zebrafish and medaka, are increasingly gaining popularity in basic research and disease modeling as a useful alternative to rodent model organisms. However, the tracking options for fish within a facility are rather limited. In this study, we present an aquatic species tracking database, Zebrabase, developed in our zebrafish research and breeding facility that represents a practical and scalable solution and an intuitive platform for scientists, fish managers, and caretakers, in both small and large facilities. Zebrabase is a scalable, cross-platform fish tracking database developed especially for fish research facilities. Nevertheless, this platform can be easily adapted for a wide variety of aquatic model organisms housed in tanks. It provides sophisticated tracking, reporting, and management functions that help keep animal-related records well organized, including a QR code functionality for tank labeling. The implementation of various user roles ensures a functional hierarchy and customized access to specific functions and data. In addition, Zebrabase makes it easy to personalize rooms and racks, and its advanced statistics and reporting options make it an excellent tool for creating periodic reports of animal usage and productivity. Communication between the facility and the researchers can be streamlined by the database functions. Finally, Zebrabase also features an interactive breeding history and a smart interface with advanced visualizations and intuitive color coding that accelerate the processes.

摘要

小型鱼类物种,如斑马鱼和青鳉,作为啮齿动物模式生物的一种有用替代品,在基础研究和疾病建模中越来越受欢迎。然而,在设施内对鱼类的追踪选择相当有限。在本研究中,我们展示了一个在我们的斑马鱼研究和养殖设施中开发的水生物种追踪数据库Zebrabase,它为大小设施中的科学家、鱼类管理人员和饲养员提供了一个实用且可扩展的解决方案以及一个直观的平台。Zebrabase是一个可扩展的跨平台鱼类追踪数据库,专为鱼类研究设施开发。尽管如此,该平台可以轻松适用于饲养在水箱中的各种水生模式生物。它提供了复杂的追踪、报告和管理功能,有助于使动物相关记录井井有条,包括用于水箱标记的二维码功能。各种用户角色的实施确保了功能层次结构以及对特定功能和数据的定制访问。此外,Zebrabase便于对房间和架子进行个性化设置,其先进的统计和报告选项使其成为创建动物使用情况和生产力定期报告的出色工具。数据库功能可以简化设施与研究人员之间的沟通。最后,Zebrabase还具有交互式繁殖历史以及带有先进可视化和直观颜色编码的智能界面,可加速相关流程。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f4b/6277078/81156803d4de/fig-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f4b/6277078/81156803d4de/fig-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f4b/6277078/81156803d4de/fig-1.jpg

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本文引用的文献

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Appropriate rearing density in domesticated zebrafish to avoid masculinization: links with the stress response.养殖斑马鱼时适宜的饲养密度以避免雄性化:与应激反应的关联
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Electronic individual identification of zebrafish using radio frequency identification (RFID) microtags.
斑马鱼Kit配体与促红细胞生成素协同作用,促进红细胞生成。
Blood Adv. 2020 Dec 8;4(23):5915-5924. doi: 10.1182/bloodadvances.2020001700.
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The effect of stocking densities on reproductive performance in laboratory zebrafish (Danio rerio).养殖密度对实验室斑马鱼(Danio rerio)繁殖性能的影响。
Zebrafish. 2011 Sep;8(3):141-6. doi: 10.1089/zeb.2011.0688. Epub 2011 Aug 19.
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Zebrafish. 2010 Jun;7(2):189-97. doi: 10.1089/zeb.2009.0648.
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