Lin Sen-Sen, Li Fang-Fang, Sun Li, Fan Wei, Gu Ming, Zhang Lu-Yong, Qin Song, Yuan Sheng-Tao
New Drug Screen Center, China Pharmaceutical University, Nanjing 210009, China.
New Drug Screen Center, China Pharmaceutical University, Nanjing 210009, China.
Chin J Nat Med. 2016 Mar;14(3):203-9. doi: 10.1016/S1875-5364(16)30017-6.
Marsdenia tenacissima, a traditional Chinese medicine, is long been used to treat various diseases including asthma, cancer, trachitis, tonsillitis, pharyngitis, cystitis, and pneumonia. Although Marsdenia tenacissima has been demonstrated to have strong anti-tumor effects against primary tumors, its effect on cancer metastasis remains to be defined, and the molecular mechanism underlying the anti-metastatic effect is unknown. In the present study, we investigated the effects of XAP (an extract of Marsdenia tenacissima) on A549 lung cancer cell migration and explored the role of CCR5-CCL5 axis in the anti-metastatic effects of XAP. Our resutls showed that XAP inhibited A549 lung cancer cell migration and invasion in a dose-dependent manner. The protein levels of CCR5, but not CCR9 and CXCR4, were decreased by XAP. The secretion of CCL5, the ligand of CCR5, was reduced by XAP. XAP down-regulated Rho C expression and FAK phosphorylation. In conclusion, XAP inhibited A549 cell migration and invasion through down-regulation of CCR5-CCL5 axis, Rho C, and FAK.
通关藤，一种传统中药，长期以来被用于治疗多种疾病，包括哮喘、癌症、气管炎、扁桃体炎、咽炎、膀胱炎和肺炎。尽管通关藤已被证明对原发性肿瘤具有强大的抗肿瘤作用，但其对癌症转移的影响仍有待确定，且抗转移作用的分子机制尚不清楚。在本研究中，我们研究了XAP（通关藤提取物）对A549肺癌细胞迁移的影响，并探讨了CCR5-CCL5轴在XAP抗转移作用中的作用。我们的结果表明，XAP以剂量依赖性方式抑制A549肺癌细胞的迁移和侵袭。XAP降低了CCR5的蛋白水平，但未降低CCR9和CXCR4的蛋白水平。XAP减少了CCR5的配体CCL5的分泌。XAP下调了Rho C表达和FAK磷酸化。总之，XAP通过下调CCR5-CCL5轴、Rho C和FAK来抑制A549细胞的迁移和侵袭。
