Nayak Sunil, Roberts Adam, Bires Kristofer, Tallarida Christopher S, Kim Erin, Wu Michael, Rawls Scott M
aCenter for Substance Abuse Research bDepartment of Pharmacology, Temple University School of Medicine, Philadelphia, Pennsylvania, USA.
Behav Pharmacol. 2016 Sep;27(6):556-8. doi: 10.1097/FBP.0000000000000236.
Planarians spend less time in light versus dark environments. We hypothesized that planarians withdrawn from cocaine or ethanol would spend even less time in the light than drug-naive planarians and that a benzodiazepine would inhibit this response. Planarians pretreated in cocaine or ethanol were placed at the midline of a Petri dish containing spring water that was split evenly into dark and light compartments. Planarians withdrawn from cocaine (1, 10, 100 μmol/l) or ethanol (0.01%) spent less time in the light compartment than water controls; however, this withdrawal response to cocaine (100 μmol/l) or ethanol (0.01%) was abolished by clorazepate (0-100 μmol/l). These data suggest that planarians, similar to rodents, show benzodiazepine-sensitive, anxiogenic-like responses during cocaine or alcohol withdrawal.
涡虫在明亮环境中停留的时间比在黑暗环境中少。我们假设，与未接触过药物的涡虫相比，戒断可卡因或乙醇的涡虫在明亮环境中停留的时间会更少，并且苯二氮䓬会抑制这种反应。将预先用可卡因或乙醇处理过的涡虫放置在装有泉水的培养皿中线处，该培养皿被均匀地分为黑暗和明亮两个隔室。从可卡因（1、10、100 μmol/l）或乙醇（0.01%）中戒断的涡虫在明亮隔室中停留的时间比用水处理的对照组少；然而，氯氮䓬（0 - 100 μmol/l）消除了对可卡因（100 μmol/l）或乙醇（0.01%）的这种戒断反应。这些数据表明，涡虫与啮齿动物相似，在可卡因或酒精戒断期间表现出对苯二氮䓬敏感的、类似焦虑的反应。
