Van Saene H K, Van Putte J C, Van Saene J J, Van de Gronde T W, Van Warmerdam E G
Department of Medical Microbiology, University of Liverpool, UK.
Epidemiol Infect. 1989 Apr;102(2):231-8. doi: 10.1017/s0950268800029903.
Sinks in a new long-stay hospital (LSH) were cultured weekly during 4 consecutive months to evaluate the microbial profile before and after occupancy of the hospital. From the elderly patients admitted to the patient care rooms oral and rectal specimens were collected to examine the contribution of the patients' flora to the sink contamination. Isolates were typed biochemically, serologically and by susceptibility pattern. Before occupancy Gram-negative bacilli were not isolated. Once the elderly patients, who were highly colonized on admission, occupied their rooms identical strains gradually contaminated the sinks. Escherichia coli, Klebsiella, Pseudomonas and Acinetobacter species were the major correlating strains. The mean concentration of the correlating isolates was higher in throat and intestines compared to the mean concentration of the non-correlating strains. These strains seem to have a greater chance to be shed and then transferred via the hands of personnel to sinks. This report shows that the major route of environmental contamination is from patient carriers to sinks, and not the reverse way.
在一家新建的长期住院医院（LSH）中，连续4个月每周对水槽进行培养，以评估医院投入使用前后的微生物状况。从入住病房的老年患者身上采集口腔和直肠标本，以检查患者菌群对水槽污染的影响。分离菌株通过生化、血清学和药敏模式进行分型。在投入使用前未分离出革兰氏阴性杆菌。一旦入院时高度定植的老年患者入住病房，相同菌株逐渐污染水槽。大肠杆菌、克雷伯菌、假单胞菌和不动杆菌属是主要的相关菌株。与非相关菌株的平均浓度相比，相关分离株在咽喉和肠道中的平均浓度更高。这些菌株似乎更有可能脱落，然后通过工作人员的手转移到水槽中。本报告表明，环境污染的主要途径是从患者携带者到水槽，而非相反。
