Charpin C, Andrac L, Habib M C, Vacheret H, Lavaut M N, Xerri L, Toga M
Department of Pathology, Timone Hospital, Marseille, France.
Gynecol Oncol. 1989 Apr;33(1):9-22. doi: 10.1016/0090-8258(89)90595-7.
Immunocytochemical assay (ICAs) were performed on frozen sections from human endometrial samples (n = 89) including normal endometrium, decidua, hyperplasia with and without atypia, and carcinomas. Monoclonal antiestrogen receptor (ER), anti-laminin (Lam), anti-type IV collagen (Coll IV), and anti-Ki67 were applied with avidin-biotin-peroxidase complex or peroxidase-antiperoxidase complex. The results of the ICAs were evaluated through a computerized system of image analysis referred to as SAMBA. It was shown that this system provided for an accurate reliable and reproducible analysis of ICAs in tissue sections. It is concluded that this multiparametric and standardized method of analysis of ICAs can further be applied in correlations with clinical and biochemical data.
对89份人类子宫内膜样本的冰冻切片进行免疫细胞化学分析（ICA），这些样本包括正常子宫内膜、蜕膜、伴或不伴异型增生的增生组织以及癌组织。使用抗雌激素受体（ER）、抗层粘连蛋白（Lam）、抗IV型胶原（Coll IV）单克隆抗体以及抗Ki67抗体，采用抗生物素蛋白-生物素-过氧化物酶复合物或过氧化物酶-抗过氧化物酶复合物。通过一个称为SAMBA的计算机图像分析系统对ICA结果进行评估。结果表明，该系统能对组织切片中的ICA进行准确、可靠且可重复的分析。得出结论，这种多参数标准化的ICA分析方法可进一步应用于与临床和生化数据的相关性研究。
