亚马逊指甲花（Onychopetalum amazonicum R.E.Fr.）精油的化学成分与抗菌活性

Chemical composition and antimicrobial activity of the essential oils of Onychopetalum amazonicum R.E.Fr.

作者信息

de Lima Bruna R, da Silva Felipe M A, Soares Elzalina R, de Almeida Richardson A, da Silva Filho Francinaldo A, Pereira Junior Raimundo C, Hernandez Tasco Álvaro J, Salvador Marcos J, Koolen Hector H F, de Souza Afonso D L, Pinheiro Maria L B

机构信息

a Departamento de Química , Universidade Federal do Amazonas , Manaus , Brazil.

b Instituto de Biologia , Universidade Estadual de Campinas , Campinas , Brazil.

出版信息

Nat Prod Res. 2016 Oct;30(20):2356-9. doi: 10.1080/14786419.2016.1163691. Epub 2016 Mar 31.

DOI:10.1080/14786419.2016.1163691

PMID:27033169

Abstract

The essential oils from leaves, twigs and trunk bark of Onychopetalum amazonicum R.E. Fr. (Annonaceae), obtained by hydrodistillation, were analysed by GC and GC-MS, and also were evaluated for in vitro antimicrobial activity. Forty-one compounds, which correspond to 75.0-92.2% of the oil components, were identified. Major compounds were sesquiterpenes, including (E)-caryophyllene, caryophyllene oxide, spathulenol, α-gurjunene, allo-aromadendrene and α-epi-cadinol. The oils were evaluated for antimicrobial activities against four bacteria strains and five pathogenic fungi. The oil of the trunk bark exhibited good activity against Staphylococcus epidermidis ATCC 12228, Escherichia coli ATCC 10538 and Kocuria rhizophila ATCC 9341, with a minimal inhibitory concentration of 62.5 μg/mL. The essential oil composition and the antimicrobial evaluation are reported for the first time for the genus Onychopetalum.

摘要

通过水蒸馏法从亚马逊爪瓣番荔枝（番荔枝科）的叶子、嫩枝和树干树皮中提取的精油，采用气相色谱（GC）和气相色谱-质谱联用（GC-MS）进行分析，并对其体外抗菌活性进行评估。共鉴定出41种化合物，占油成分的75.0%-92.2%。主要化合物为倍半萜类，包括（E）-石竹烯、石竹烯氧化物、匙叶桉油烯醇、α-古芸烯、别香树烯和α-表杜松醇。评估了这些精油对四种细菌菌株和五种致病真菌的抗菌活性。树干树皮的精油对表皮葡萄球菌ATCC 12228、大肠杆菌ATCC 10538和嗜根考克氏菌ATCC 9341表现出良好的活性，最低抑菌浓度为62.5μg/mL。首次报道了爪瓣番荔枝属的精油成分和抗菌评估情况。