Shakeri Abolfazl, Akhtari Javad, Soheili Vahid, Taghizadeh Seyedeh Faezeh, Sahebkar Amirhossein, Shaddel Rezvan, Asili Javad
Student Research Committee, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Immunogenetic Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran; Department of Nanobiomedicine, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Microb Pathog. 2017 Aug;109:39-44. doi: 10.1016/j.micpath.2017.05.022. Epub 2017 May 16.
Chemical composition and biological (antimicrobial, antioxidant and cytotoxic) activities of essential oils (EO) obtained from the aerial parts of Glycyrrhiza triphylla Fisch. & C.A.Mey (G. triphylla) were evaluated in the present study. The EO was isolated and analyzed using gas chromatography-mass spectrometry (GC-MS). Fifty-five compounds representing 99.3% of the total oil composition were identified. Major components of the oil were β-caryophyllene (25.4%), limonene (16.7%), β-myrcene (16.0%) and α-humulene (4.4%). The oil composition was dominated by the presence of sesquiterpene hydrocarbons comprising 43.6% of the total oil. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the oil were determined against eight bacterial strains and one fungus. The EO showed a good antibacterial activity against both Gram-positive and Gram-negative bacteria. The most susceptible strain was Micrococcus luteus (MIC = 2.7 μg/mL, MBC = 43.6 μg/mL). The antioxidant potential of the EO was examined using DPPH and β-carotene/linoleic acid (BCB) assays. The oil was considerably active in the DPPH assay (IC = 100.40 ± 0.03 μg/mL). Moreover, in vitro cytotoxic activity was assessed against six cancer cell lines using MTT assay. The EO showed no significant cytotoxic activity. In light of the present findings, G. triphylla oil may deserves to be further investigated for its potential therapeutic effects and also as a natural preservative in food industry.
本研究对从甘草（Glycyrrhiza triphylla Fisch. & C.A.Mey）地上部分提取的挥发油（EO）的化学成分及生物（抗菌、抗氧化和细胞毒性）活性进行了评估。采用气相色谱 - 质谱联用（GC - MS）对挥发油进行分离和分析。共鉴定出55种化合物，占总油成分的99.3%。该油的主要成分是β-石竹烯（25.4%）、柠檬烯（16.7%）、β-月桂烯（16.0%）和α-葎草烯（4.4%）。油成分以倍半萜烃为主，占总油的43.6%。测定了该油对8种细菌菌株和1种真菌的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。该挥发油对革兰氏阳性菌和革兰氏阴性菌均表现出良好的抗菌活性。最敏感的菌株是藤黄微球菌（MIC = 2.7μg/mL，MBC = 43.6μg/mL）。采用DPPH和β-胡萝卜素/亚油酸（BCB）法检测了挥发油的抗氧化潜力。该油在DPPH法中具有相当高的活性（IC = 100.40±0.03μg/mL）。此外，使用MTT法评估了其对6种癌细胞系的体外细胞毒性活性。该挥发油未表现出显著的细胞毒性活性。根据目前的研究结果，甘草挥发油因其潜在的治疗作用以及作为食品工业中的天然防腐剂，可能值得进一步研究。
