马的血清胰岛素浓度：储存与处理的影响

Serum insulin concentration in horses: Effect of storage and handling.

作者信息

Carslake Harry, Karikoski Ninja, Pinchbeck Gina, McGowan Catherine

机构信息

Institute of Ageing and Chronic Disease and Infection and Global Health, Faculty of Health and Life Sciences, University of Liverpool, Neston, Wirral CH64 7TE, UK.

Department of Equine and Small Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland.

出版信息

Vet J. 2016 May;211:94-6. doi: 10.1016/j.tvjl.2016.02.016. Epub 2016 Mar 4.

DOI:10.1016/j.tvjl.2016.02.016

PMID:27033594

Abstract

Serum insulin concentration is commonly measured during investigation of suspected endocrinopathic disease in horses, but immediate analysis is frequently unavailable. The aim of this study was to examine the effect of storing samples at room temperature for 72 h as serum and as whole blood, compared to immediate separation and freezing. Samples from 14 horses were evaluated. Correlation was excellent for all comparisons (≥0.992). Bland-Altman plots revealed a negative bias (mean difference 2.16 µIU/mL) in samples stored as whole blood compared to serum, but this difference was not considered clinically significant. At two commonly used diagnostic cut-offs, there was no effect of storage on result. This study indicates that storage at room temperature for 72 h, either as serum or whole blood, has minimal effect on measured serum insulin concentration in horses.

摘要

在对疑似患有内分泌疾病的马匹进行检查时，通常会检测血清胰岛素浓度，但往往无法立即进行分析。本研究的目的是检验与立即分离并冷冻相比，将样本在室温下保存72小时作为血清和全血的效果。对14匹马的样本进行了评估。所有比较的相关性都非常好（≥0.992）。布兰德-奥特曼图显示，与血清相比，作为全血保存的样本存在负偏差（平均差异为2.16µIU/mL），但这种差异在临床上不被认为具有显著意义。在两个常用的诊断临界值下，保存对结果没有影响。本研究表明，在室温下保存72小时，无论是作为血清还是全血，对马匹血清胰岛素浓度的测量影响极小。