Department of Biomedical Sciences, College of Veterinary Medicine and Agriculture, Addis Ababa University, Bishoftu, Ethiopia.
Vet Med Sci. 2024 Mar;10(2):e1368. doi: 10.1002/vms3.1368.
Delays between blood collection and analysis are inevitable, and samples are always stored in the refrigerator. The current study aimed to evaluate the stability of serum total cholesterol (TC), triglycerides (TG), total protein (TP), albumin and urea (URA) in horses and oxen after storage at -20°C.
Sera from apparently healthy 20 male horses and 20 oxen were obtained and aliquots of serum were divided into 3 portions. The first tube was used for baseline (T0) measurement of analyte values, whereas the other two tubes, T1 and T2, were stored at -20°C for 1 and 2 months, respectively, and analyte measurement was done.
Results showed that the stability of TP (g/dL), URA (mg/dL) and TC (mg/dL) in oxen was statistically significant (p < 0.05). In horses, the stability of URA (mg/dL), TP (g/dL) and TG (mg/dL) were also statistically significant (p < 0.05). Additionally, URA and TC in oxen exceed TE following measurement at T2 and TG in horses following measurement at T1 and T2.
Laboratories should consider the storage temperature and time for specific analytes among animals. Therefore, stability studies at various storage temperatures and times are recommended to fully validate the stability of the analytes.
从采血到分析之间必然存在时间延迟，且样本通常都储存在冰箱中。本研究旨在评估马和牛血清总胆固醇（TC）、甘油三酯（TG）、总蛋白（TP）、白蛋白和尿素（URA）在-20°C 储存条件下的稳定性。
采集 20 匹雄性马和 20 头公牛的血清样本，等分血清并分为 3 管。第一管用于基线（T0）分析物值测量，而其他两管（T1 和 T2）分别在-20°C 下储存 1 个月和 2 个月，然后进行分析物测量。
结果表明，牛的 TP（g/dL）、URA（mg/dL）和 TC（mg/dL）的稳定性具有统计学意义（p<0.05）。在马中，URA（mg/dL）、TP（g/dL）和 TG（mg/dL）的稳定性也具有统计学意义（p<0.05）。此外，牛的 URA 和 TC 在 T2 测量时超过 TE，马的 TG 在 T1 和 T2 测量时超过 TE。
实验室应考虑动物特定分析物的储存温度和时间。因此，建议在各种储存温度和时间下进行稳定性研究，以充分验证分析物的稳定性。
