Swiss Institute of Equine Medicine, Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of Bern, and Agroscope, Bern, Switzerland.
Swiss Institute of Equine Medicine, Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of Bern, and Agroscope, Bern, Switzerland.
Vet J. 2021 Aug;274:105706. doi: 10.1016/j.tvjl.2021.105706. Epub 2021 Jun 9.
Sorbitol dehydrogenase (SDH) activity is one of the most sensitive and specific markers for hepatocellular injury in horses, but its reported lability makes it impractical for use in many clinical settings. To date, stability of SDH in equine samples has only been evaluated in a limited number of studies in serum samples of horses with activities within reference intervals. The objective of the study was to determine pre-analytical stability of equine SDH activity in heparinized plasma stored at different temperatures for up to 72 h. Twenty client-owned horses admitted to a veterinary teaching hospital for any reason were included in the study. Blood samples collected in lithium-heparin tubes were immediately centrifuged and SDH activity was analyzed within 1 h of collection (T0). Aliquots of plasma were stored at room temperature, 4 °C and -20 °C and SDH activity was re-analyzed after 4 h (T4), 24 h (T24) and 72 h (T72). A significant difference from values measured at T0 was found for samples stored at room temperature (P = 0.022) and -20 °C (P < 0.001), but not at 4 °C. The activity of SDH was within ±20% of that measured at T0 for all samples under all temperature conditions stored for 4 h, and for all samples stored at 4 °C for 24 h. Bland-Altman plots revealed narrow limits of agreement at T4 for all storage temperatures and at T24 for samples stored at 4 °C. The mean absolute percentage error and 95th percentile of the absolute percentage error were lower for samples stored at 4 °C than those stored at room temperature or -20 °C. The activity of SDH has adequate stability for 4 h regardless of storage temperature and 24 h if stored at 4 °C across a wide range of values. Knowledge of the pre-analytical stability of SDH may permit its broader use in assessing hepatic disorders in horses.
山梨醇脱氢酶（SDH）活性是马肝细胞损伤最敏感和特异的标志物之一，但由于其报道的不稳定性，使其在许多临床环境中不实用。迄今为止，只有在少数研究中评估了马 SDH 在血清样本中的稳定性，这些研究中的马匹的活性均在参考区间内。本研究的目的是确定肝素化血浆中马 SDH 活性在不同温度下储存长达 72 小时的预分析稳定性。将因任何原因被收入兽医教学医院的 20 匹患畜纳入研究。采集到含锂肝素管中的血液样本立即离心，在采集后 1 小时内分析 SDH 活性（T0）。将等分血浆样本储存在室温、4°C 和-20°C 下，并在 4 小时（T4）、24 小时（T24）和 72 小时（T72）时重新分析 SDH 活性。与 T0 时测量的值相比，室温（P = 0.022）和-20°C（P < 0.001）储存的样本有显著差异，但 4°C 储存的样本无显著差异。在所有温度条件下储存 4 小时的所有样本以及在 4°C 储存 24 小时的所有样本中，SDH 的活性均在 T0 测量值的±20%范围内。Bland-Altman 图显示，在所有储存温度下的 T4 时和在 4°C 储存时的 T24 时，均具有较窄的一致性界限。与在室温或-20°C 储存的样本相比，在 4°C 储存的样本的平均绝对百分比误差和 95%绝对百分比误差较小。SDH 的活性在 4°C 下储存 4 小时时具有足够的稳定性，在 4°C 下储存 24 小时时，无论储存温度如何，其稳定性都足够。了解 SDH 的预分析稳定性可能使其更广泛地用于评估马的肝障碍。
