Centre for Biofuels, Biotechnology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Industrial Estate PO, Trivandrum 695019, India.
Centre for Biofuels, Biotechnology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Industrial Estate PO, Trivandrum 695019, India.
Bioresour Technol. 2016 Aug;213:216-221. doi: 10.1016/j.biortech.2016.03.114. Epub 2016 Mar 23.
Harvesting of the micro alga Chlorococcum sp. R-AP13 through autoflocculation, chemical flocculants or by change in medium pH was evaluated. Surface charge of algal cells changed in response to the method used and affected flocculation efficiency. While aluminum sulfate and FeCl3 supported 87% and 92% efficiency, auto flocculation could recover 75% of biomass in 10min. Maximum efficiency (94%) was obtained with change in medium pH from 8.5 to 12.0 achieved through addition of 40mgl(-1) of NaOH. Since high concentrations of FeCl3 and AlSO4 were toxic to the cells, flocculation induced by pH change may be considered the most effective strategy. Residual medium after flocculation could be reused efficiently for algal cultivation, minimizing the demand for fresh water.
通过自絮凝、化学絮凝剂或改变介质 pH 值来评估微藻 Chlorococcum sp. R-AP13 的收获情况。藻细胞的表面电荷会随着所使用方法的变化而变化，并影响絮凝效率。硫酸铝和 FeCl3 的支持效率分别为 87%和 92%，而自絮凝在 10min 内可回收 75%的生物质。通过添加 40mgl(-1)的 NaOH 将介质 pH 值从 8.5 改变到 12.0 时，可获得最大效率（94%）。由于高浓度的 FeCl3 和 AlSO4 对细胞有毒，因此可以考虑通过 pH 值变化诱导的絮凝是最有效的策略。絮凝后残留的培养基可以有效地再用于藻类培养，从而最大限度地减少对淡水的需求。
