Park Seeun, Erdogan Sedef, Hwang Dahyun, Hwang Seonwook, Han Eun Hye, Lim Young-Hee
Department of Integrated Biomedical and Life Sciences, Graduate School, Korea University.
Biol Pharm Bull. 2016 Jun 1;39(6):1060-8. doi: 10.1248/bpb.b16-00158. Epub 2016 Apr 2.
Alopecia is an important issue that can occur in people of all ages. Recent studies show that bee venom can be used to treat certain diseases including rheumatoid arthritis, neuralgia, and multiple sclerosis. In this study, we investigated the preventive effect of bee venom on alopecia, which was measured by applying bee venom (0.001, 0.005, 0.01%) or minoxidil (2%) as a positive control to the dorsal skin of female C57BL/6 mice for 19 d. Growth factors responsible for hair growth were analyzed by quantitative real-time PCR and Western blot analysis using mice skins and human dermal papilla cells (hDPCs). Bee venom promoted hair growth and inhibited transition from the anagen to catagen phase. In both anagen phase mice and dexamethasone-induced catagen phase mice, hair growth was increased dose dependently compared with controls. Bee venom inhibited the expression of SRD5A2, which encodes a type II 5α-reductase that plays a major role in the conversion of testosterone into dihydrotestosterone. Moreover, bee venom stimulated proliferation of hDPCs and several growth factors (insulin-like growth factor 1 receptor (IGF-1R), vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF)2 and 7) in bee venom-treated hDPCs dose dependently compared with the control group. In conclusion, bee venom is a potentially potent 5α-reductase inhibitor and hair growth promoter.
脱发是一个在所有年龄段人群中都可能出现的重要问题。最近的研究表明，蜂毒可用于治疗某些疾病，包括类风湿性关节炎、神经痛和多发性硬化症。在本研究中，我们研究了蜂毒对脱发的预防作用，通过将蜂毒（0.001%、0.005%、0.01%）或作为阳性对照的米诺地尔（2%）涂抹于雌性C57BL/6小鼠背部皮肤19天来进行测量。使用小鼠皮肤和人真皮乳头细胞（hDPCs），通过定量实时PCR和蛋白质免疫印迹分析来分析负责头发生长的生长因子。蜂毒促进头发生长，并抑制从生长期到退行期的转变。在生长期小鼠和地塞米松诱导的退行期小鼠中，与对照组相比，毛发生长均呈剂量依赖性增加。蜂毒抑制了SRD5A2的表达，SRD5A2编码一种II型5α-还原酶，该酶在睾酮转化为二氢睾酮的过程中起主要作用。此外，与对照组相比，蜂毒剂量依赖性地刺激了hDPCs的增殖以及蜂毒处理的hDPCs中几种生长因子（胰岛素样生长因子-1受体（IGF-1R）、血管内皮生长因子（VEGF）、成纤维细胞生长因子（FGF）2和7）的表达。总之，蜂毒是一种潜在的强效5α-还原酶抑制剂和头发生长促进剂。
