Li Hui, He Hongjuan, Wang Zhengxuan, Cai Jixiang, Sun Bonan, Wu Qiong, Zhang Yue, Zhou Gengyong, Yang Lin
Department of Food Science and Engineering, School of Chemical Engineering and Technology, Harbin Institute of Technology, 73 Huanghe Road, Harbin 150090, China.
School of Life Science and Biotechnology, Harbin Institute of Technology, Harbin 150001, China.
Gene. 2016 Jul 10;585(2):256-64. doi: 10.1016/j.gene.2016.03.052. Epub 2016 Mar 31.
To elucidate the effects of rice protein on the detoxification and antioxidant defense via the Nuclear factor erythroid 2-related factor 2 (Nrf2) pathway, adult rats were fed casein and rice protein under cholesterol-free and -enriched dietary conditions. Nrf2 proteins and gene expressions were stimulated by rice proteins with respect to caseins accompanied by up-regulating the expression of gene encoding antioxidant and phase II detoxification in the rice protein groups. In the liver, compared with caseins, rice proteins significantly increased hepatic contents of reduced glutathione (GSH) and mRNA levels of glutamate cysteine ligase catalytic subunit (GCLC), glutamate cysteine ligase modulatory subunit (GCLM), glutathione S-transferase (GST), heme oxygenase 1 (HO-1) and
NAD(P)H: quinone oxidoreductase 1 (NQO1), whereas hepatic accumulations of reactive oxygen species (ROS) and oxidized glutathione (GSSG) were markedly depressed in adult rats fed rice proteins. The present study demonstrates the endogenous antioxidant potential of rice proteins via mechanism through which Nrf2 activation plays an essential role in inducing Phase II antioxidants/detoxification enzymes and preventing ROS generation. This novel antioxidative function of rice protein is independent of dietary cholesterol during in adult rats.
为阐明大米蛋白通过核因子红细胞2相关因子2（Nrf2）途径对解毒和抗氧化防御的影响，在无胆固醇和富含胆固醇的饮食条件下，给成年大鼠喂食酪蛋白和大米蛋白。与酪蛋白相比，大米蛋白刺激了Nrf2蛋白和基因表达，并伴随上调大米蛋白组中抗氧化剂和II期解毒相关基因的表达。在肝脏中，与酪蛋白相比，大米蛋白显著增加了还原型谷胱甘肽（GSH）的肝含量以及谷氨酸半胱氨酸连接酶催化亚基（GCLC）、谷氨酸半胱氨酸连接酶调节亚基（GCLM）、谷胱甘肽S-转移酶（GST）、血红素加氧酶1（HO-1）和NAD（P）H：醌氧化还原酶1（NQO1）的mRNA水平，而喂食大米蛋白的成年大鼠肝脏中活性氧（ROS）和氧化型谷胱甘肽（GSSG）的积累明显减少。本研究通过Nrf2激活在诱导II期抗氧化剂/解毒酶和预防ROS生成中起重要作用的机制，证明了大米蛋白的内源性抗氧化潜力。在成年大鼠中，大米蛋白的这种新型抗氧化功能与饮食胆固醇无关。
