Department of Biotechnology, Konkuk University, Chungju, 27478, South Korea.
Division of Food Bio Science, Konkuk University, Chungju, 27478, South Korea.
Adv Exp Med Biol. 2017;975 Pt 1:243-254. doi: 10.1007/978-94-024-1079-2_22.
Oxidative stress mediates the cell damage in several neurodegenerative diseases, some of which are Alzheimer's disease (AD), multiple sclerosis and Parkinson's disease (PD). In this study, we investigated whether the taurine-rich cuttlefish extract could exert a protective effect on damaged human neuroblastoma SH-SY5Y cells induced by hydrogen peroxide (HO). Our results revealed that pre-treatment with cuttlefish extract effectively increased the cell viability by protecting the cells from intracellular reactive oxygen species (ROS) induced by HO exposure. Furthermore, apoptosis related proteins Bcl-2 and Bax were investigated by western-blot analysis and results indicated that cuttlefish extract promoted the expression of anti-apoptotic Bcl-2 protein while inhibiting the expression of pro-apoptotic Bax protein. Therefore, cuttlefish extract containing the ability of scavenging excessive ROS, the capacity of anti-oxidative stress, could be employed in neurodegenerative disease prevention. In conclusion, the results suggest that cuttlefish extract could be used as a potential candidate for preventing several human neurodegenerative and other disorders caused by oxidative stress.
氧化应激介导了几种神经退行性疾病中的细胞损伤，其中一些是阿尔茨海默病（AD）、多发性硬化症和帕金森病（PD）。在这项研究中，我们研究了富含牛磺酸的乌贼提取物是否对过氧化氢（HO）诱导的受损人神经母细胞瘤 SH-SY5Y 细胞发挥保护作用。我们的结果表明，乌贼提取物预处理通过保护细胞免受 HO 暴露引起的细胞内活性氧（ROS）的伤害，有效地增加了细胞活力。此外，通过 Western blot 分析研究了与细胞凋亡相关的蛋白 Bcl-2 和 Bax，结果表明乌贼提取物促进了抗凋亡 Bcl-2 蛋白的表达，同时抑制了促凋亡 Bax 蛋白的表达。因此，富含牛磺酸的乌贼提取物具有清除过多 ROS 的能力、抗氧化应激的能力，可用于预防神经退行性疾病。总之，这些结果表明，乌贼提取物可用作预防由氧化应激引起的几种人类神经退行性疾病和其他疾病的潜在候选药物。
