Besar S S, Kelly S W, Greenhalgh P A
Electronic Engineering Laboratories, University of Kent, Canterbury, UK.
J Biomed Eng. 1989 Mar;11(2):151-6. doi: 10.1016/0141-5425(89)90127-1.
A simple, inexpensive fibre optic spectrophotometric cell has been developed for clinical use. The system may employ one of two indicator reagents for the measurement: phenol red and BDH universal indicator. The spectrophotometer uses two ultrabright LED sources and a PIN diode photodetector. The fibre is of the plastic type with a core diameter of 1.0 mm. Two alternative analytical methods are available: electronic or computer processing. In the case of phenol red the measuring range using computer processing is between 6.0 and 8.0 pH units, with an accuracy of 0.015 pH units. The range for electronic circuit processing is from 6.8 to 8.0 pH units with an accuracy of 0.02 pH units. Using a BDH universal indicator, the range for computer processing is between 5.5 and 8.5 pH units with an accuracy of 0.05 pH units, while with electronic processing the range is between 6.0 and 8.0 pH units with an accuracy of 0.03 pH units. A description of the optoelectronics, an analysis of the indicator reagents and the calibration procedure are presented here, together with some example results.
已开发出一种用于临床的简单、廉价的光纤分光光度测量池。该系统可使用两种指示试剂之一进行测量：酚红和BDH通用指示剂。分光光度计使用两个超亮LED光源和一个PIN二极管光电探测器。光纤为塑料类型，芯径为1.0毫米。有两种可供选择的分析方法：电子处理或计算机处理。对于酚红，使用计算机处理时的测量范围为6.0至8.0 pH单位，精度为0.015 pH单位。电子电路处理的范围为6.8至8.0 pH单位，精度为0.02 pH单位。使用BDH通用指示剂时，计算机处理的范围为5.5至8.5 pH单位，精度为0.05 pH单位，而电子处理的范围为6.0至8.0 pH单位，精度为0.03 pH单位。本文介绍了光电子学、指示试剂分析和校准程序，以及一些示例结果。
