Jiang You-Ming, Li Xiao-Juan, Meng Zhen-Zhi, Liu Yue-Yun, Zhao Hong-Bo, Li Na, Yan Zhi-Yi, Ma Qing-Yu, Zhang Han-Ting, Chen Jia-Xu
School of Preclinical Medicine, Beijing University of Chinese Medicine, No. 11, Beisanhuan Donglu, Chaoyang, Beijing 100029, China.
Department of Behavioral Medicine & Psychiatry, West Virginia University Health Sciences Center, Morgantown, WV 26506-9137, USA.
Evid Based Complement Alternat Med. 2016;2016:1238426. doi: 10.1155/2016/1238426. Epub 2016 Mar 2.
Background. Compared with antidepressant activity of Xiaoyaosan, the role of Xiaoyaosan in anxiety has been poorly studied. Objective. To observe the effects of Xiaoyaosan on anxiety-like behavior induced by chronic immobilization stress (CIS) and further explore whether these effects were related to CRF1R signaling. Methods. Adult male SD rats were randomly assigned to five groups (n = 12): the nonstressed control group, vehicle-treated (saline, p.o.) group, Xiaoyaosan-treated (3.854 g/kg, p.o.) group, vehicle-treated (surgery) group, and antalarmin-treated (surgery) group. Artificial cerebrospinal fluid (0.5 μL/side) or CRF1R antagonist antalarmin (125 ng/0.5 μL, 0.5 μL/side) was bilaterally administered into the basolateral amygdala in the surgery groups. Except for the nonstressed control group, the other four groups were exposed to CIS (14 days, 3 h/day) 30 minutes after treatment. On days 15 and 16, all animals were subjected to the elevated plus-maze (EPM) and novelty suppressed feeding (NSF) test. We then examined the expression of CRF1R, pCREB, and BDNF in the amygdala. Results. Chronic pretreatment with Xiaoyaosan or antalarmin significantly reversed elevated anxiety-like behavior and the upregulated level of CRF1R and BDNF in the amygdala of stressed rats. pCREB did not differ significantly among the groups. Conclusions. These results suggest that Xiaoyaosan exerts anxiolytic-like effects in behavioral tests and the effects may be related to CRF1R signaling in the amygdala.
背景。与逍遥散的抗抑郁活性相比，逍遥散在焦虑方面的作用研究较少。目的。观察逍遥散对慢性束缚应激（CIS）诱导的焦虑样行为的影响，并进一步探讨这些影响是否与促肾上腺皮质激素释放因子1受体（CRF1R）信号通路有关。方法。成年雄性SD大鼠随机分为五组（n = 12）：非应激对照组、溶剂处理（生理盐水，口服）组、逍遥散处理（3.854 g/kg，口服）组、溶剂处理（手术）组和安他拉敏处理（手术）组。手术组双侧杏仁核基底外侧核注射人工脑脊液（0.5 μL/侧）或CRF1R拮抗剂安他拉敏（125 ng/0.5 μL，0.5 μL/侧）。除非应激对照组外，其他四组在处理后30分钟接受CIS（14天，每天3小时）。在第15天和第16天，所有动物进行高架十字迷宫（EPM）和新奇抑制摄食（NSF）试验。然后检测杏仁核中CRF1R、磷酸化环磷腺苷反应元件结合蛋白（pCREB）和脑源性神经营养因子（BDNF）的表达。结果。逍遥散或安他拉敏的慢性预处理显著逆转了应激大鼠杏仁核中升高的焦虑样行为以及CRF1R和BDNF上调水平。各组间pCREB无显著差异。结论。这些结果表明，逍遥散在行为试验中发挥抗焦虑样作用，且该作用可能与杏仁核中的CRF1R信号通路有关。
