School of Preclinical Medicine, Beijing University of Chinese Medicine, No. 11, Beisanhuan Donglu, Chaoyang, Beijing 100029, China ; School of Preclinical Medicine, Youjiang Medical University for Nationalities, Baise, Guangxi 533000, China.
School of Preclinical Medicine, Beijing University of Chinese Medicine, No. 11, Beisanhuan Donglu, Chaoyang, Beijing 100029, China ; Department of Basic Theory in Chinese Medicine, Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China.
Evid Based Complement Alternat Med. 2013;2013:297154. doi: 10.1155/2013/297154. Epub 2013 Dec 29.
Xiaoyaosan (XYS) decoction is a famous prescription which can protect nervous system from stress and treat liver stagnation and spleen deficiency syndrome (LSSDS). In this experiment, we observed the effect of XYS decoction on chronic immobilization stress (CIS) induced learning and memory deficit in rats from behaviors and changes of proteins in hippocampus. We used XYS decoction to treat CIS induced learning and memory deficit in rats with rolipram as positive control, used change of body weight and behavioral tests to determine whether the rats have LSSDS and have learning and memory deficit or not. We used Western blotting to determine the content of postsynaptic density protein 95 (PSD-95) and synaptophysin (SYP) in hippocampus. Results showed that XYS could improve the situation of slow weight gain induced by CIS, improve the ability of learning and memory, reverse the symptom of liver stagnation and spleen deficiency syndrome (LSSDS) in rats, and increase the levels of PSD-95 and SYP on the hippocampal nerve synapses. These findings suggested that XYS decoction may be helpful in reversing CIS induced learning and memory deficit by increasing the levels of PSD-95 and SYP on the hippocampal nerve synapses and improving synaptic plasticity.
逍遥散（XYS）是一种著名的方剂，可保护神经系统免受压力，治疗肝郁脾虚证（LSSDS）。在这项实验中，我们观察了 XYS 汤对慢性束缚应激（CIS）诱导的大鼠学习和记忆缺陷的影响，从行为和海马体蛋白质的变化来观察。我们用 XYS 汤治疗 CIS 诱导的学习和记忆缺陷的大鼠，用罗利普兰作为阳性对照，用体重变化和行为测试来确定大鼠是否有 LSSDS 和学习记忆缺陷。我们用 Western blot 来确定海马体中突触后密度蛋白 95（PSD-95）和突触小体相关蛋白（SYP）的含量。结果表明，XYS 可改善 CIS 引起的体重增长缓慢的情况，提高学习和记忆能力，逆转大鼠肝郁脾虚证（LSSDS）的症状，并增加海马神经突触上 PSD-95 和 SYP 的水平。这些发现表明，XYS 汤可能通过增加海马神经突触上 PSD-95 和 SYP 的水平，改善突触可塑性，有助于逆转 CIS 诱导的学习和记忆缺陷。
