Ewen C, Hendry J H
Department of Radiobiology, Paterson Institute for Cancer Research, Christie Hospital, Manchester, United Kingdom.
Radiat Res. 1989 Apr;118(1):169-79.
A short-term colony assay for renal tubule epithelium has been developed. Uranyl nitrate (UN) is a heavy metal nephrotoxin that induces acute tubule necrosis followed by a large compensatory increase in the rate of cell proliferation in the nephron. UN was used to precipitate latent damage following renal irradiation. Using a subcapsular colony count at 14 days after unilateral irradiation, a single-dose cell survival curve was obtained with a D0 of 4.2 +/- 0.3 Gy. High-dose irradiation of an exteriorized kidney resulted in a survival curve which was biphasic, with a plateau in survival between 18 and 40 Gy. Subtraction of this plateau level from all the survival data gave D0 values of 2.5 +/- 0.2 Gy (data analyzed between 7.5 and 16 Gy) or 2.0 +/- 0.2 Gy (over range 12-16 Gy). The D0 value obtained at 20 months after bilateral (or unilateral) kidney irradiation, without the use of UN, was 2.9 +/- 1.1 Gy (over range 10-14 Gy).
已开发出一种用于肾小管上皮的短期集落测定法。硝酸铀酰（UN）是一种重金属肾毒素，可诱导急性肾小管坏死，随后肾单位中的细胞增殖速率大幅代偿性增加。UN被用于引发肾脏照射后的潜在损伤。通过单侧照射后14天的被膜下集落计数，获得了单剂量细胞存活曲线，D0为4.2±0.3 Gy。对离体肾脏进行高剂量照射得到的存活曲线呈双相，在18至40 Gy之间的存活有一个平台期。从所有存活数据中减去该平台期水平，得到的D0值为2.5±0.2 Gy（在7.5至16 Gy之间分析数据）或2.0±0.2 Gy（在12 - 16 Gy范围内）。在双侧（或单侧）肾脏照射后20个月，未使用UN时获得的D0值为2.9±1.1 Gy（在10至14 Gy范围内）。
