Yu X, Ren H, Liu T, Yong M, Zhong H
Clin Exp Obstet Gynecol. 2016;43(1):75-81.
To study the potential pathogenesis of endometriosis (EMs) in an area of estrogen receptors (ERs) and tyrosine kinase receptor type B (TrkB) expressions in tissues from patients with EMs.
The authors examined the expressions of ERα, ERβ, TrkB, brain-derived neurotrophic factor (BDNF), and SGPL1 in tissues with EMs, using real-time PCR, western blot, and immunohistochemistry.
ERα and SGPL1 were mainly expressed in eutopic endometrium than that in ectopic endometrium of patients with ovarian endometriosis (p < 0.05), while ERβ, BDNF, and TrkB were adverse, mainly detected in ectopic endometrium of the same patients with EMs (p < 0.01 and p < 0.05 ) by real-time PCR and western blot. ERβ, ERα, TrkB, and SGPL1 proteins were mainly expressed in eutopic endometrium of proliferative phase with EMs than that in eutopic endometrium of secretory phase (p < 0.05 ). TrkB, BDNF, and SGPL1 were not found in endometrium of proliferative or secretory phase in control group.
ERβ expressed in cytoplasm may mediate pathogenesis of EMs.
在子宫内膜异位症（EMs）患者组织中雌激素受体（ERs）和B型酪氨酸激酶受体（TrkB）表达区域研究EMs的潜在发病机制。
作者采用实时定量聚合酶链反应、蛋白质免疫印迹法和免疫组织化学法检测了EMs组织中ERα、ERβ、TrkB、脑源性神经营养因子（BDNF）和鞘氨醇磷酸酯酶1（SGPL1）的表达。
实时定量聚合酶链反应和蛋白质免疫印迹法检测显示，与卵巢子宫内膜异位症患者的异位内膜相比，ERα和SGPL1主要在其在位内膜中表达（p<0.05），而ERβ、BDNF和TrkB则相反，主要在同一EMs患者的异位内膜中检测到（p<0.01和p<0.05）。ERβ、ERα、TrkB和SGPL1蛋白在增殖期EMs在位内膜中的表达高于分泌期在位内膜（p<0.05）。对照组增殖期或分泌期子宫内膜中未检测到TrkB、BDNF和SGPL1。
细胞质中表达的ERβ可能介导EMs的发病机制。
