Tascioglu Tulin, Metin Ozge Karakas, Aydin Yildiz, Sakiroglu Muhammet, Akan Kadir, Uncuoglu Ahu Altinkut
Department of Bioengineering, Faculty of Engineering, Marmara University, 34722, Istanbul, Turkey.
TÜBİTAK, Marmara Research Center, Genetic Engineering and Biotechnology Institute, 41470, Kocaeli, Turkey.
Biochem Genet. 2016 Aug;54(4):421-437. doi: 10.1007/s10528-016-9729-x. Epub 2016 Apr 5.
Bread wheat (Triticum aestivum L.) gene pool was analyzed with 117 microsatellite markers scattered throughout A, B, and D genomes. Ninety microsatellite markers were giving 1620 polymorphic alleles in 55 different bread wheat genotypes. These genotypes were found to be divided into three subgroups based on Bayesian model and Principal component analysis. The highest polymorphism information content value for the markers resides on A genome was estimated for wmc262 marker located on 4A chromosome with the polymorphism information content value of 0.960. The highest polymorphism information content value (0.954) among the markers known to be located on B genome was realized for wmc44 marker located on 1B chromosome. The highest polymorphism information content value for the markers specific to D genome was found in gwm174 marker located on 5D chromosome with the polymorphism information content value of 0.948. The presence of linkage disequilibrium between 81 pairwise SSR markers reside on the same chromosome was tested and very limited linkage disequilibrium was observed. The results confirmed that the most distant genotype pairs were as follows Ceyhan-99-Behoth 6, Gerek 79-Douma 40989, and Karahan-99-Douma 48114.
利用分布于A、B和D基因组的117个微卫星标记对面包小麦（Triticum aestivum L.）基因库进行了分析。90个微卫星标记在55个不同的面包小麦基因型中产生了1620个多态性等位基因。基于贝叶斯模型和主成分分析，这些基因型被分为三个亚组。位于4A染色体上的wmc262标记在A基因组上的标记中具有最高的多态性信息含量值，为0.960。已知位于B基因组上的标记中，位于1B染色体上的wmc44标记具有最高的多态性信息含量值（0.954）。在位于5D染色体上的gwm174标记中发现了D基因组特异性标记的最高多态性信息含量值，为0.948。对位于同一条染色体上的81对SSR标记之间的连锁不平衡进行了检测，观察到的连锁不平衡非常有限。结果证实，距离最远的基因型对如下：Ceyhan-99-Behoth 6、Gerek 79-Douma 40989和Karahan-99-Douma 48114。
