Qi Jian-Yong, Wang Lei, Gu Dong-Sheng, Guo Li-Heng, Zhu Wei, Zhang Min-Zhou
Intensive Care Laboratory, Guangdong Province Hospital of Chinese Medicine, The 2nd Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510120, China.
Department of Rheumatology, The 3rd Affiliated Hospital of Southern Medical University, Guangzhou, 510630, China.
Chin J Integr Med. 2018 Aug;24(8):613-620. doi: 10.1007/s11655-016-2448-7. Epub 2016 Mar 21.
To observe the in vivo effect of Danlou Tablet (, DLT) on myocardial ischemia and reperfusion (I/R) injury.
DLT effects were evaluated in mouse heart preparation using 30-min coronary occlusion followed by 24-h reperfusion and compared among sham group (n=6), I/R group (n=8), IPC group (ischemia preconditioning, n=6) and DLT group (I/R with DLT pretreatment for 3 days, 750 mg•kg•day, n=8). The effects of DLT were characterized in infarction size (IS) compared with risk region (RR) and left ventricle using the Evans blue/triphenyltetrazolium chloride double dye staining method in vivo. Furthermore, the dose-dependent effect of DLT on I/R injury was evaluated by double staining method. Five different concentrations of DLT (0.625, 1.25, 2.5, 5 and 10 g•kg•day) were chosen in this study, and dose-response curve of DLT was obtained on these data.
The ratio of IS to left ventricle was significantly smaller in the DLT and IPC groups than the I/R group (P<0.05 or P<0.01), the ratio of IS to RR was also reduced in the DLT and IPC groups (P<0.01), while there were no differences in RR among the four groups (P>0.05). Experiments showed incidence of arrhythmias was reduced in the DLT group (P<0.01). Furthermore, DLT produced a dose-dependent inhibitory effect with a half maximal inhibitory concentration of 1.225 g•kg•day.
Our research concluded that DLT was effective in reducing I/R injury in mice, and provided experimental supports for the clinical use of DLT.
观察丹蒌片（DLT）对心肌缺血再灌注（I/R）损伤的体内作用。
采用小鼠心脏制备模型，通过30分钟冠状动脉阻断及24小时再灌注来评估DLT的作用，并在假手术组（n = 6）、I/R组（n = 8）、缺血预处理组（IPC，n = 6）和DLT组（I/R模型，DLT预处理3天，750毫克•千克•天，n = 8）之间进行比较。在体内使用伊文思蓝/氯化三苯基四氮唑双重染色法，以梗死面积（IS）与危险区域（RR）及左心室的比值来表征DLT的作用。此外，通过双重染色法评估DLT对I/R损伤的剂量依赖性作用。本研究选择了五种不同浓度的DLT（0.625、1.25、2.5、5和10克•千克•天），并根据这些数据得到DLT的剂量-反应曲线。
DLT组和IPC组的IS与左心室的比值显著小于I/R组（P < 0.05或P < 0.01），DLT组和IPC组的IS与RR的比值也降低（P < 0.01），而四组之间的RR无差异（P > 0.05）。实验表明DLT组心律失常的发生率降低（P < 0.01）。此外，DLT产生剂量依赖性抑制作用，半数最大抑制浓度为1.225克•千克•天。
我们的研究得出结论，DLT可有效减轻小鼠的I/R损伤，为DLT的临床应用提供了实验依据。
