Sgoutas D S, Hammarstrom M
Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia 30322.
Transplantation. 1989 Apr;47(4):668-70. doi: 10.1097/00007890-198904000-00019.
This study compares two recently introduced radioimmunoassay kits involving specific monoclonal antibodies to cyclosporine. One kit (Sandimmun) involved 3H-labeled CsA (3H-CsA) as tracer and the other (CY-CLO-Trac-SP) involved a 125I-labeled conjugated derivative of CsA. The kits were nearly equivalent in method performance characteristics. They produced superimposed standard curves and equivalent values to transplanted patient samples. Concentrations of CsA determined by either kit were apparently equivalent to values measured by high-performance liquid chromatography, suggesting that the specific monoclonal antibodies used with the kits detect in trough blood mainly native CsA. The 125I-labeled CsA, when compared with the 3H-CsA alternative, increased the sensitivity and precision, decreased the turnaround time, and provided a technically efficient and conveniently capable method of replacing HPLC for measuring native CsA.
本研究比较了两种最近推出的涉及环孢素特异性单克隆抗体的放射免疫分析试剂盒。一种试剂盒（山地明）采用3H标记的环孢素A（3H-CsA）作为示踪剂，另一种（CY-CLO-Trac-SP）采用125I标记的环孢素A结合衍生物。这两种试剂盒在方法性能特征方面几乎相当。它们生成的标准曲线相互重叠，对移植患者样本得出的值也相当。两种试剂盒测定的环孢素A浓度显然与高效液相色谱法测得的值相当，这表明试剂盒中使用的特异性单克隆抗体在谷值血中主要检测的是天然环孢素A。与3H-CsA替代方法相比，125I标记的环孢素A提高了灵敏度和精密度，缩短了周转时间，并提供了一种技术上高效且方便可行的方法来替代高效液相色谱法测定天然环孢素A。
