沙特阿拉伯西部流行的流感病毒对神经氨酸酶抑制剂的敏感性。
Susceptibility of influenza viruses circulating in Western Saudi Arabia to neuraminidase inhibitors.
作者信息
Tolah Ahmed M, Azhar Esam I, Hashem Anwar M
机构信息
Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Fahd Medical Research Center, Jeddah, Kingdom of Saudi Arabia. E-mail.
出版信息
Saudi Med J. 2016 Apr;37(4):461-5. doi: 10.15537/smj.2016.4.14837.
OBJECTIVES
To investigate the sensitivity of circulating influenza viruses in Western Saudi Arabia to neuraminidase inhibitors (NAIs); mainly, zanamivir and oseltamivir.
METHODS
Respiratory samples were collected from patients presenting with respiratory symptoms to King Abdulaziz University Hospital, Jeddah, Kingdom of Saudi Arabia (KSA) between September 2013 and October 2014. All samples were tested prospectively by real-time reverse-transcription polymerase chain reaction for influenza A and B viruses. Positive samples were then inoculated on Madin-Darby Canine Kidney (MDCK) cells and isolated viruses were examined for their sensitivity to NAIs using fluorescent neuraminidase inhibition assay.
RESULTS
Out of 406 tested samples, 25 samples (6.2%) were positive for influenza A/pdmH1N1 virus, one sample (0.25%) was positive for influenza A/H3N2 virus, and 7 samples (1.7%) were positive for influenza B Yamagata-like virus. Screening of isolated influenza A and B viruses (9 out of 33) for their sensitivity to NAIs showed no significant resistance to available NAIs.
CONCLUSION
Our results show that circulating influenza viruses in Jeddah are still sensitive to NAIs.
目的
调查沙特阿拉伯西部流行的流感病毒对神经氨酸酶抑制剂（NAIs）的敏感性；主要是扎那米韦和奥司他韦。
方法
2013年9月至2014年10月期间，从沙特阿拉伯王国吉达阿卜杜勒阿齐兹国王大学医院出现呼吸道症状的患者中采集呼吸道样本。所有样本均通过实时逆转录聚合酶链反应对甲型和乙型流感病毒进行前瞻性检测。阳性样本随后接种到犬肾传代细胞（MDCK）上，并使用荧光神经氨酸酶抑制试验检测分离出的病毒对NAIs的敏感性。
结果
在406份检测样本中，25份（6.2%）甲型/ pdmH1N1流感病毒呈阳性，1份（0.25%）甲型/H3N2流感病毒呈阳性，7份（1.7%）乙型山形流感病毒呈阳性。对分离出的甲型和乙型流感病毒（33份中的9份）进行NAIs敏感性筛查，结果显示对现有NAIs无明显耐药性。
结论
我们的结果表明，吉达流行的流感病毒对NAIs仍然敏感。
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