Myles Ian A, Reckhow Jensen D, Williams Kelli W, Sastalla Inka, Frank Karen M, Datta Sandip K
Bacterial Pathogenesis Unit, Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.
Department of Laboratory Medicine, National Institutes of Health Clinical Center, Bethesda, MD, USA.
BMC Microbiol. 2016 Apr 6;16:60. doi: 10.1186/s12866-016-0684-9.
Commensal Gram-negative (CGN) microbiota have been identified on human skin by DNA sequencing; however, methods to reliably culture viable Gram-negative skin organisms have not been previously described.
Through the use of selective antibiotics and minimal media we developed methods to culture CGN from skin swabs. We identified several previously uncharacterized CGN at the species level by optimizing growth conditions and limiting the inhibitory effects of nutrient shock, temperature, and bacterial competition, factors that may have previously limited CGN isolation from skin cultures.
Our protocol will permit future functional studies on the influences of CGN on skin homeostasis and disease.
通过DNA测序已在人类皮肤上鉴定出共生革兰氏阴性（CGN）微生物群；然而，此前尚未描述可靠培养活的革兰氏阴性皮肤微生物的方法。
通过使用选择性抗生素和基本培养基，我们开发了从皮肤拭子中培养CGN的方法。我们通过优化生长条件并限制营养休克、温度和细菌竞争的抑制作用，在物种水平上鉴定了几种先前未被表征的CGN，这些因素可能以前限制了从皮肤培养物中分离CGN。
我们的方案将允许未来对CGN对皮肤稳态和疾病影响的功能研究。
