Wang Pan, Li Huijuan, Zhang Xingjiang, Wang Xiaoxun, Sun Wenwen, Zhang Xiaoya, Chi Baiyi, Go Yuyo, Chan Xi Hui Felicia, Wu Jianxin, Huang Qing
Skin Health and Cosmetic Development & Evaluation Laboratory, China Pharmaceutical University, Nanjing, 211198, Jiangsu, China.
Department of Engineering Science, University of Oxford, Parks Road, Oxford, OX1 3PJ, UK.
Nat Commun. 2025 Mar 31;16(1):3085. doi: 10.1038/s41467-025-58377-2.
Skin microecology involves a dynamic equilibrium among the host, microbiome, and internal/external environments. This equilibrium, shaped by multifactorial interactions, reflects individual specificity and diversity. Creating a replicable in vitro skin microecological model is highly challenging. Here, we introduce a mimicked stratum corneum microecology model (SCmic). It uses light cured crosslinked hydrogels as a scaffold and moisture source, and nonviable epidermal cells as the main nutrient. This setup establishes a suitable, stable, and reproducible microecology for microbiome colonization. Notably, it replicates the normal/oily skin microbiota with no significant differences from the original native microbiota at the genus level. Simultaneously, we have developed a standardized human skin microbiota model (Hcm), featuring seven dominant strains that form a representative microbial community. The models provide highly convenient approaches for exploring the intricate mutual interactions among skin microecology, influence of microbiota on skin health, and metabolism of chemical substances by microbiota.
皮肤微生态涉及宿主、微生物群以及内部/外部环境之间的动态平衡。这种由多因素相互作用形成的平衡反映了个体特异性和多样性。创建一个可复制的体外皮肤微生态模型极具挑战性。在此，我们引入一种模拟角质层微生态模型（SCmic）。它使用光固化交联水凝胶作为支架和水分来源，并使用无活力的表皮细胞作为主要营养物质。这种设置为微生物群定殖建立了一个适宜、稳定且可重复的微生态。值得注意的是，它能复制正常/油性皮肤微生物群，在属水平上与原始天然微生物群无显著差异。同时，我们开发了一种标准化的人类皮肤微生物群模型（Hcm），其具有七种优势菌株，形成一个代表性的微生物群落。这些模型为探索皮肤微生态之间复杂的相互作用、微生物群对皮肤健康的影响以及微生物群对化学物质的代谢提供了极为便利的方法。
