Wang Ailiang, Zhang Wei, Jin Meng, Zhang Jianbo, Li Sheng, Tong Feng, Zhou Yanbing
Department of General Surgery, The Affiliated Hospital of Medical College of Qingdao University, Qingdao, Shandong 266003, P.R. China.
Department of General Surgery, The Affiliated Hospital of Jining Medical University, Jining, Shandong 272000, P.R. China.
Mol Med Rep. 2016 May;13(5):4151-8. doi: 10.3892/mmr.2016.5087. Epub 2016 Apr 5.
Epstein‑Barr virus (EBV) infection is associated with the development of T cell lymphoma, nasopharyngeal cancer (NPC), and EBV‑associated gastric cancer (EBVaGC). This study assessed the expression of the EBV‑associated proteins latent membrane protein 1 (LMP1) and BamHI‑A rightward frame 1 (BARF1) in NPC and EBVaGC tissue specimens and determined their association with clinicopathological data, microvessel density (MVD) and micro‑lymphatic vessel density (MLVD). This study collected 600 gastric cancer and 75 NPC tissue samples. EBV infection was assessed using in situ hybridization, and LMP1 and BARF1 expression was assessed using immunohistochemistry. The levels of MVD and MLVD were assessed using immunostaining of vascular endothelial growth factor (VEGF)‑C, CD34, and lymphatic vessel endothelial receptor 1 (LYVE‑1). Among the 600 gastric cancer cases, 30 were positive for EBV infection, which was shown to be associated with the age of patients (P=0.073), tumor differentiation (P<0.0001), tumor location (P<0.0001) and lymph node metastasis (P<0.0001). In these 30 EBVaGC cases, only one case was weakly positive for LMP1, but 17 cases were BARF1 positive. BARF1 expression was associated with lymph node metastasis of EBVaGC and the level of MLVD. Furthermore, 61 (81%) of 75 NPC patients were EBV positive, among which 38 cases were LMP‑1 positive (62.3%) and LMP1 expression was associated with tumor‑node‑metastasis stage (P=0.011) and lymph node metastasis (P=0.041). MLVD was significantly higher in LMP1‑positive cases than LMP1‑negative cases. There were only 8 (13.3%) cases positive for BARF1 expression. In conclusion, EBV infection exhibits a role in gastric cancer and NPC development; however, expression of EBV‑associated proteins LMP1 and BARF1 have differential functions during tumorigenesis of these two types of cancer.
爱泼斯坦-巴尔病毒（EBV）感染与T细胞淋巴瘤、鼻咽癌（NPC）和EBV相关胃癌（EBVaGC）的发生有关。本研究评估了NPC和EBVaGC组织标本中EBV相关蛋白潜伏膜蛋白1（LMP1）和BamHI-A向右框1（BARF1）的表达，并确定了它们与临床病理数据、微血管密度（MVD）和微淋巴管密度（MLVD）的关系。本研究收集了600份胃癌组织样本和75份NPC组织样本。采用原位杂交评估EBV感染情况，采用免疫组织化学评估LMP1和BARF1的表达。采用血管内皮生长因子（VEGF）-C、CD34和淋巴管内皮受体1（LYVE-1）免疫染色评估MVD和MLVD水平。在600例胃癌病例中，30例EBV感染呈阳性，结果显示这与患者年龄（P=0.073）、肿瘤分化程度（P<0.0001）、肿瘤位置（P<0.0001）和淋巴结转移（P<0.0001）有关。在这30例EBVaGC病例中，只有1例LMP1弱阳性，但17例BARF1阳性。BARF1表达与EBVaGC的淋巴结转移及MLVD水平有关。此外，75例NPC患者中有61例（81%）EBV阳性，其中38例LMP-1阳性（62.3%），LMP1表达与肿瘤-淋巴结-转移分期（P=0.011）和淋巴结转移（P=0.041）有关。LMP1阳性病例的MLVD显著高于LMP1阴性病例。BARF1表达阳性的病例仅有8例（13.3%）。总之，EBV感染在胃癌和NPC发生中发挥作用；然而，EBV相关蛋白LMP1和BARF1在这两种癌症的肿瘤发生过程中具有不同的功能。
