Marriott A C, Ward V K, Nuttall P A
NERC Institute of Virology, Oxford, United Kingdom.
Virology. 1989 Apr;169(2):341-5. doi: 10.1016/0042-6822(89)90159-1.
The complete nucleotide sequence of the S RNA segment of Sandfly Fever Sicilian (SFS) virus (Phlebovirus, Bunyaviridae) was determined from overlapping cDNA clones and by primer extension. The RNA is 1746 nucleotides in length and has two large open reading frames (ORF), one of which (24.8 kDa) is viral-complementary in sense, and the other (30.4 kDa) is in the viral sense. This ambisense genome arrangement has been seen in another member of the Phlebovirus genus, Punta Toro (PT) virus (T. Ihara, H. Akashi, and D. H. L. Bishop, 1984, Virology 136, 293-306), but not in representatives of either the Bunyavirus or Hantavirus genera of the Bunyaviridae. Comparison of the predicted amino acid sequences for SFS virus with the recognized products of PT S RNA (T. Ihara, Y. Matsuura, and D. H. L. Bishop, 1985, Virology 147, 317-325; H. A. Overton, T. Ihara, and D. H. L. Bishop, 1987, Virology 157, 338-350) indicated that the 24.8-kDa ORF encodes the nucleoprotein (N) of SFS virus, and the 30.4-kDa ORF codes for a nonstructural protein (NSs). Subgenomic messenger RNAs, from which these two proteins are presumably translated, were detected in virus-infected cells.
通过重叠cDNA克隆和引物延伸法，测定了西西里白蛉热（SFS）病毒（白蛉病毒属，布尼亚病毒科）S RNA片段的完整核苷酸序列。该RNA长度为1746个核苷酸，有两个大的开放阅读框（ORF），其中一个（24.8 kDa）在病毒互补链上有义，另一个（30.4 kDa）在病毒链上有义。这种双义基因组排列在白蛉病毒属的另一个成员——蓬塔托罗（PT）病毒中也有发现（T. Ihara、H. Akashi和D. H. L. Bishop，1984年，《病毒学》136卷，293 - 306页），但在布尼亚病毒科的布尼亚病毒属或汉坦病毒属的代表病毒中未发现。将SFS病毒预测的氨基酸序列与PT S RNA的已知产物进行比较（T. Ihara、Y. Matsuura和D. H. L. Bishop；1985年，《病毒学》147卷，317 - 325页；H. A. Overton、T. Ihara和D. H. L. Bishop，1987年，《病毒学》157卷，338 - 350页）表明，24.8 kDa的ORF编码SFS病毒的核蛋白（N），30.4 kDa的ORF编码一种非结构蛋白（NSs）。在病毒感染的细胞中检测到了可能翻译这两种蛋白质的亚基因组信使RNA。
