Gerbaud S, Vialat P, Pardigon N, Wychowski C, Girard M, Bouloy M
Unité de Virologie Moléculaire, UA CNRS 545, Institut Pasteur, Paris, France.
Virus Res. 1987 Jul;8(1):1-13. doi: 10.1016/0168-1702(87)90035-9.
The complete sequence of the S segment of Germiston bunyavirus has been determined from plasmids containing S cDNA inserts. The S segment is 980 nucleotides long with the first 15 bases at the 3' end complementary to the first 15 bases at the 5' end. Three overlapping open reading frames (ORF) were identified in the viral complementary RNA strand. The first ORF codes for a polypeptide of 233 amino acids (Mr 26,600) which is the nucleoprotein N. The second ORF codes for a polypeptide of 109 amino acids (Mr 11,800) which corresponds to the NSS protein, also called p12. Following this ORF, in the same frame, a third ORF which could encode a polypeptide of 75 amino acids was identified. Such a polypeptide has not yet been detected in infected cells. The N and NSS proteins of Germiston virus were compared with the corresponding proteins of La Crosse, snowshoe hare, and Aino viruses, and show a high extent of homology.
已从含有S cDNA插入片段的质粒中确定了杰米斯顿布尼亚病毒S节段的完整序列。S节段长980个核苷酸，3'端的前15个碱基与5'端的前15个碱基互补。在病毒互补RNA链中鉴定出三个重叠的开放阅读框（ORF）。第一个ORF编码一个由233个氨基酸组成的多肽（Mr 26,600），即核蛋白N。第二个ORF编码一个由109个氨基酸组成的多肽（Mr 11,800），对应于NSS蛋白，也称为p12。在这个ORF之后，在同一阅读框中，鉴定出一个可能编码由75个氨基酸组成的多肽的第三个ORF。在受感染细胞中尚未检测到这样的多肽。将杰米斯顿病毒的N和NSS蛋白与拉克罗斯病毒、雪兔病毒和阿依努病毒的相应蛋白进行了比较，结果显示它们具有高度的同源性。
