Giebel L B, Raab K, Zöller L, Bautz E K, Darai G
Institut für Molekulare Genetik, Universität Heidelberg, FRG.
Virus Genes. 1991 Apr;5(2):111-20. doi: 10.1007/BF00571926.
The genetic characterization of a serologically Hantaan-like virus but of unknown origin (termed DX) was carried out by molecular cloning and nucleotide sequencing of the corresponding cDNA of the viral S RNA segment. The S RNA was found to be 1765 nucleotides long with 3' and 5' termini being complementary for 24 bases. The virus messenger-sense RNA contains one major open reading frame (ORF) encoding 428 amino acids or a 50 kD polypeptide. A comparison of the DX S RNA segment to those of Sapporo rat, Hantaan, Puumala/Hällnäs B1, and Prospect Hill viruses reveals 95.4, 71.3, 55.3, and 60.9% homology at the nucleotide sequence level, and 94.7, 80.1, 58.4, and 59.8% at the deduced amino acid sequence level. Thus Hantavirus strain DX is very closely related to Sapporo rat virus. We also analyzed the S RNA segments of these Hantaviruses for the presence of a second ORF encoding a potential nonstructural NSs protein. All potential second ORFs detected in the different S RNA segments differ substantially in length and position among the viruses, despite the high conservation of the nucleotide sequences and the overall structure of the nucleocapsid proteins. This suggests that the nucleocapsid protein is the only polypeptide encoded by Hantavirus S RNA segments, setting them apart from the other members of the Bunyaviridae family.
对一种血清学上类似汉坦病毒但来源不明（称为DX）的病毒进行了基因特征分析，方法是对病毒S RNA片段的相应cDNA进行分子克隆和核苷酸测序。发现S RNA长1765个核苷酸，其3'和5'末端有24个碱基互补。病毒信使意义RNA包含一个主要开放阅读框（ORF），编码428个氨基酸或一个50 kD的多肽。将DX S RNA片段与札幌大鼠病毒、汉坦病毒、普马拉/黑尔纳斯B1病毒和展望山病毒的片段进行比较，在核苷酸序列水平上的同源性分别为95.4%、71.3%、55.3%和60.9%，在推导氨基酸序列水平上分别为94.7%、80.1%、58.4%和59.8%。因此，汉坦病毒株DX与札幌大鼠病毒密切相关。我们还分析了这些汉坦病毒的S RNA片段，以确定是否存在编码潜在非结构NSs蛋白的第二个ORF。尽管核苷酸序列高度保守且核衣壳蛋白的整体结构相似，但在不同S RNA片段中检测到的所有潜在第二个ORF在病毒间的长度和位置上有很大差异。这表明核衣壳蛋白是汉坦病毒S RNA片段编码的唯一多肽，这使其与布尼亚病毒科的其他成员有所不同。
