Shruthi Sureshan C, Habeeb S K M
Department of Bioinformatics, School of Bioengineering, SRM University, Kattankulathur 603203, Tamil Nadu, India.
Appl Transl Genom. 2015 Oct 25;7:2-12. doi: 10.1016/j.atg.2015.10.003. eCollection 2015 Dec.
MicroRNAs (miRNAs) are a class of small RNAs, evolutionarily conserved endogenous non-coding RNAs that regulate their target mRNA expression by either inactivating or degrading mRNA genes; thus playing an important role in the growth and development of an organism. Maruca vitrata is an insect pest of leguminous plants like pigeon pea, cowpea and mung bean and is pantropical. In this study, we perform BLAST on all known miRNAs against the transcriptome data of M. vitrata and thirteen miRNAs were identified. These miRNAs were characterised and their target genes were identified using TargetScan and were functionally annotated using FlyBase. The importance of the structure of pre-miRNA in the Drosha activity led to study the backbone torsion angles of predicted pre-miRNAs (mvi-miR-9751, mvi-miR-649-3p, mvi-miR-4057 and mvi-miR-1271) to identify various nucleotide triplets that contribute to the variation of torsion angle values at various structural motifs of a pre-miRNA.
微小RNA（miRNA）是一类小RNA，是进化上保守的内源性非编码RNA，通过使mRNA基因失活或降解来调节其靶mRNA的表达；因此在生物体的生长发育中发挥重要作用。豆野螟是豇豆、豇豆和绿豆等豆科植物的一种害虫，分布于泛热带地区。在本研究中，我们将所有已知的miRNA与豆野螟的转录组数据进行比对，鉴定出13个miRNA。对这些miRNA进行了表征，并使用TargetScan鉴定了它们的靶基因，使用FlyBase进行了功能注释。前体miRNA结构在Drosha活性中的重要性促使我们研究预测的前体miRNA（mvi-miR-9751、mvi-miR-649-3p、mvi-miR-4057和mvi-miR-1271）的骨架扭转角，以识别有助于前体miRNA各种结构基序处扭转角值变化的各种核苷酸三联体。
