Jackson Marisa, Tourtellotte Warren
Department of Neurology, Feinberg School of Medicine, Northwestern University, Chicago, USA.
Bio Protoc. 2014 Jan 20;4(2). doi: 10.21769/bioprotoc.1035.
The rodent superior cervical ganglion (SCG) is a useful and readily accessible source of neurons for studying the mechanisms of sympathetic nervous system (SNS) development and growth The sympathetic nervous system (SNS) of early postnatal animals undergoes a great deal of remodeling and development; thus, neurons taken from mice at this age are primed to re-grow and establish synaptic connections after removal. The stereotypic location and size of the SCG make it ideal for rapid isolation and dissociation. The protocol described here details the requirements for the dissection, culture and differentiation of SCG neurons. The protocol is suitable for culturing neurons from late embryonic gestation to approximately postnatal day 3. The culture technique discussed below utilizes glass coverslips for the microscopic examination of fixed cells.
啮齿动物的颈上神经节（SCG）是研究交感神经系统（SNS）发育和生长机制的有用且易于获取的神经元来源。出生后早期动物的交感神经系统（SNS）会经历大量重塑和发育；因此，取自这个年龄段小鼠的神经元在被移除后易于重新生长并建立突触连接。颈上神经节的固定位置和大小使其成为快速分离和解离的理想选择。此处描述的方案详细说明了颈上神经节神经元解剖、培养和分化的要求。该方案适用于培养从胚胎后期到出生后约第3天的神经元。下面讨论的培养技术利用玻璃盖玻片对固定细胞进行显微镜检查。
