• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

FoxO1在氧化应激诱导的颗粒细胞凋亡中的关键作用及葡萄籽原花青素B2的保护作用

Critical Role of FoxO1 in Granulosa Cell Apoptosis Caused by Oxidative Stress and Protective Effects of Grape Seed Procyanidin B2.

作者信息

Zhang Jia-Qing, Gao Bin-Wen, Wang Jing, Ren Qiao-Ling, Chen Jun-Feng, Ma Qiang, Zhang Zi-Jing, Xing Bao-Song

机构信息

Institute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou 450008, China.

出版信息

Oxid Med Cell Longev. 2016;2016:6147345. doi: 10.1155/2016/6147345. Epub 2016 Jan 19.

DOI:10.1155/2016/6147345
PMID:27057282
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4745910/
Abstract

Reactive oxygen species (ROS) are closely related to the follicular granulosa cell apoptosis. Grape seed procyanidin B2 (GSPB2) has been reported to possess potent antioxidant activity. However, the GSPB2-mediated protective effects and the underlying molecular mechanisms in granulosa cell apoptosis process remain unknown. In this study, we showed for the first time that GSPB2 treatment decreased FoxO1 protein level, improved granulosa cell viability, upregulated LC3-II protein level, and reduced granulosa cell apoptosis rate. Under a condition of oxidative stress, GSPB2 reversed FoxO1 nuclear localization and increased its level in cytoplasm. In addition, FoxO1 knockdown inhibited the protective effects of GSPB2 induced. Our findings suggest that FoxO1 plays a pivotal role in regulating autophagy in granulosa cells, GSPB2 exerts a potent and beneficial role in reducing granulosa cell apoptosis and inducing autophagy process, and targeting FoxO1 could be significant in fighting against oxidative stress-reduced female reproductive system diseases.

摘要

活性氧（ROS）与卵泡颗粒细胞凋亡密切相关。据报道，葡萄籽原花青素B2（GSPB2）具有强大的抗氧化活性。然而，GSPB2介导的颗粒细胞凋亡过程中的保护作用及潜在分子机制仍不清楚。在本研究中，我们首次表明，GSPB2处理降低了FoxO1蛋白水平，提高了颗粒细胞活力，上调了LC3-II蛋白水平，并降低了颗粒细胞凋亡率。在氧化应激条件下，GSPB2逆转了FoxO1的核定位并增加了其在细胞质中的水平。此外，敲低FoxO1可抑制GSPB2诱导的保护作用。我们的研究结果表明，FoxO1在调节颗粒细胞自噬中起关键作用，GSPB2在减少颗粒细胞凋亡和诱导自噬过程中发挥强大而有益的作用，靶向FoxO1在对抗氧化应激导致的女性生殖系统疾病中可能具有重要意义。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b35/4745910/3bf145b274e8/OMCL2016-6147345.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b35/4745910/75ab29b6b9b1/OMCL2016-6147345.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b35/4745910/8ab8aa6d2550/OMCL2016-6147345.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b35/4745910/3be561416c40/OMCL2016-6147345.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b35/4745910/c1d875243667/OMCL2016-6147345.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b35/4745910/b65bca968127/OMCL2016-6147345.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b35/4745910/8373c233bd5f/OMCL2016-6147345.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b35/4745910/481f60085c50/OMCL2016-6147345.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b35/4745910/8db542aa9af9/OMCL2016-6147345.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b35/4745910/3bf145b274e8/OMCL2016-6147345.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b35/4745910/75ab29b6b9b1/OMCL2016-6147345.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b35/4745910/8ab8aa6d2550/OMCL2016-6147345.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b35/4745910/3be561416c40/OMCL2016-6147345.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b35/4745910/c1d875243667/OMCL2016-6147345.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b35/4745910/b65bca968127/OMCL2016-6147345.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b35/4745910/8373c233bd5f/OMCL2016-6147345.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b35/4745910/481f60085c50/OMCL2016-6147345.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b35/4745910/8db542aa9af9/OMCL2016-6147345.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b35/4745910/3bf145b274e8/OMCL2016-6147345.009.jpg

相似文献

1
Critical Role of FoxO1 in Granulosa Cell Apoptosis Caused by Oxidative Stress and Protective Effects of Grape Seed Procyanidin B2.FoxO1在氧化应激诱导的颗粒细胞凋亡中的关键作用及葡萄籽原花青素B2的保护作用
Oxid Med Cell Longev. 2016;2016:6147345. doi: 10.1155/2016/6147345. Epub 2016 Jan 19.
2
Grape Seed Procyanidin B2 Protects Porcine Ovarian Granulosa Cells against Oxidative Stress-Induced Apoptosis by Upregulating let-7a Expression.葡萄籽原花青素 B2 通过上调 let-7a 表达来保护猪卵巢颗粒细胞免受氧化应激诱导的凋亡。
Oxid Med Cell Longev. 2019 Nov 19;2019:1076512. doi: 10.1155/2019/1076512. eCollection 2019.
3
Grape seed procyanidin B2 protects podocytes from high glucose-induced mitochondrial dysfunction and apoptosis via the AMPK-SIRT1-PGC-1α axis in vitro.体外实验中，葡萄籽原花青素B2通过AMPK-SIRT1-PGC-1α轴保护足细胞免受高糖诱导的线粒体功能障碍和细胞凋亡。
Food Funct. 2016 Feb;7(2):805-15. doi: 10.1039/c5fo01062d.
4
Grape seed procyanidin B2 ameliorates mitochondrial dysfunction and inhibits apoptosis via the AMP-activated protein kinase-silent mating type information regulation 2 homologue 1-PPARγ co-activator-1α axis in rat mesangial cells under high-dose glucosamine.葡萄籽原花青素B2通过AMP激活的蛋白激酶-沉默信息调节因子2同源物1-过氧化物酶体增殖物激活受体γ共激活因子-1α轴改善高剂量氨基葡萄糖作用下大鼠系膜细胞的线粒体功能障碍并抑制细胞凋亡。
Br J Nutr. 2015 Jan 14;113(1):35-44. doi: 10.1017/S000711451400347X. Epub 2014 Nov 18.
5
Grape seed procyanidin b2 inhibits human aortic smooth muscle cell proliferation and migration induced by advanced glycation end products.葡萄籽原花青素B2抑制晚期糖基化终产物诱导的人主动脉平滑肌细胞增殖和迁移。
Biosci Biotechnol Biochem. 2011;75(9):1692-7. doi: 10.1271/bbb.110194. Epub 2011 Sep 7.
6
Effects of Grape Seed Extract and Proanthocyanidin B2 on In Vitro Proliferation, Viability, Steroidogenesis, Oxidative Stress, and Cell Signaling in Human Granulosa Cells.葡萄籽提取物和原花青素 B2 对人颗粒细胞体外增殖、活力、甾体生成、氧化应激和细胞信号转导的影响。
Int J Mol Sci. 2019 Aug 28;20(17):4215. doi: 10.3390/ijms20174215.
7
PIMT prevents the apoptosis of endothelial cells in response to glycated low density lipoproteins and protective effects of grape seed procyanidin B2.PIMT 可防止内皮细胞对糖化低密度脂蛋白的凋亡反应，以及葡萄种子原花青素 B2 的保护作用。
PLoS One. 2013 Jul 26;8(7):e69979. doi: 10.1371/journal.pone.0069979. Print 2013.
8
Protective mechanism of FSH against oxidative damage in mouse ovarian granulosa cells by repressing autophagy.FSH 通过抑制自噬对小鼠卵巢颗粒细胞氧化损伤的保护机制。
Autophagy. 2017 Aug 3;13(8):1364-1385. doi: 10.1080/15548627.2017.1327941. Epub 2017 Jun 9.
9
Hepatoprotective Effects of Grape Seed Procyanidin B2 in Rats With Carbon Tetrachloride-induced Hepatic Fibrosis.葡萄籽原花青素B2对四氯化碳诱导的肝纤维化大鼠的肝保护作用
Altern Ther Health Med. 2015;21 Suppl 2:12-21.
10
Restoration of Mimecan Expression by Grape Seed Procyanidin B2 Through Regulation of Nuclear Factor-kappaB in Mice With Diabetic Nephropathy.葡萄籽原花青素B2通过调节核因子-κB恢复糖尿病肾病小鼠中的 mimecan 表达
Iran J Kidney Dis. 2016 Sep;10(5):325-331.

引用本文的文献

1
Melatonin protects chicken follicular granulosa cells against oxidative damage-induced apoptosis via inhibiting FOXO1 signaling pathway.褪黑素通过抑制FOXO1信号通路保护鸡卵泡颗粒细胞免受氧化损伤诱导的凋亡。
Poult Sci. 2025 Jun 24;104(9):105472. doi: 10.1016/j.psj.2025.105472.
2
Targeting programmed cell death with natural products: a potential therapeutic strategy for diminished ovarian reserve and fertility preservation.利用天然产物靶向程序性细胞死亡：一种针对卵巢储备功能减退和生育力保存的潜在治疗策略。
Front Pharmacol. 2025 May 29;16:1546041. doi: 10.3389/fphar.2025.1546041. eCollection 2025.
3
Effect of , and Gene Pyramiding on Litter Traits of Goats.

本文引用的文献

1
Reactive oxygen species in ovarian physiology.卵巢生理学中的活性氧物种
Reprod Med Biol. 2005 Mar 7;4(1):31-44. doi: 10.1007/BF03016135. eCollection 2005 Mar.
2
FOXO1 is regulated by insulin and IGF1 in pituitary gonadotropes.在垂体促性腺细胞中，FOXO1受胰岛素和胰岛素样生长因子1调控。
Mol Cell Endocrinol. 2015 Apr 15;405:14-24. doi: 10.1016/j.mce.2015.02.006. Epub 2015 Feb 9.
3
Critical role of prohibitin in endothelial cell apoptosis caused by glycated low-density lipoproteins and protective effects of grape seed procyanidin B2.
、和基因聚合对山羊产仔性状的影响。 需注意，原文中“Effect of, and Gene Pyramiding”这里的逗号之间应该是有具体基因名称缺失了，导致译文读起来不太完整准确。
Animals (Basel). 2025 May 8;15(10):1358. doi: 10.3390/ani15101358.
4
Proanthocyanidins delaying the premature ovarian insufficiency through regulatory sirt1-p53-p21 signaling pathway in female germline stem cells.原花青素通过调节雌性生殖干细胞中的sirt1-p53-p21信号通路延缓卵巢早衰。
J Ovarian Res. 2025 May 10;18(1):97. doi: 10.1186/s13048-025-01661-y.
5
Effect of 1-DNJ on Oxidative Stress-Induced Apoptosis in Porcine Ovarian GCs Through Modulation of the PERK-ATF4/MFN2 Signaling Pathway.1-脱氧野尻霉素通过调控PERK-ATF4/MFN2信号通路对氧化应激诱导的猪卵巢颗粒细胞凋亡的影响
Antioxidants (Basel). 2025 Apr 11;14(4):456. doi: 10.3390/antiox14040456.
6
Anti-Mullerian Hormone Induces and Genes Expression in Mouse Ovary.抗苗勒管激素诱导小鼠卵巢中的基因表达。
Curr Pharm Biotechnol. 2025;26(6):872-877. doi: 10.2174/0113892010293250240917143811.
7
The multiple actions of grape and its polyphenols on female reproductive processes with an emphasis on cell signalling.葡萄及其多酚对女性生殖过程的多种作用及其对细胞信号转导的影响。
Front Endocrinol (Lausanne). 2024 Jan 4;14:1245512. doi: 10.3389/fendo.2023.1245512. eCollection 2023.
8
Acne Transcriptomics: Fundamentals of Acne Pathogenesis and Isotretinoin Treatment.痤疮转录组学：痤疮发病机制和异维 A 酸治疗的基础。
Cells. 2023 Nov 10;12(22):2600. doi: 10.3390/cells12222600.
9
Effect of prolactin on cytotoxicity and oxidative stress in ovine ovarian granulosa cells.催乳素对绵羊卵巢颗粒细胞细胞毒性和氧化应激的影响。
PeerJ. 2023 Jul 10;11:e15629. doi: 10.7717/peerj.15629. eCollection 2023.
10
Impact of vitamin B12 on the reproductive health of women with sickle cell disease: a narrative review.维生素B12对镰状细胞病女性生殖健康的影响：一项叙述性综述
Reprod Fertil. 2023 Jul 1;4(3). doi: 10.1530/RAF-23-0015.
抑制素在糖化低密度脂蛋白引起的内皮细胞凋亡中的关键作用及葡萄籽原花青素B2的保护作用
J Cardiovasc Pharmacol. 2015 Jan;65(1):13-21. doi: 10.1097/FJC.0000000000000157.
4
Autophagy as a pro-death pathway.自噬作为一种促死亡途径。
Immunol Cell Biol. 2015 Jan;93(1):35-42. doi: 10.1038/icb.2014.85. Epub 2014 Oct 21.
5
Proteomic analysis of aorta and protective effects of grape seed procyanidin B2 in db/db mice reveal a critical role of milk fat globule epidermal growth factor-8 in diabetic arterial damage.主动脉蛋白质组学分析及葡萄籽原花青素 B2 对 db/db 小鼠的保护作用揭示了乳脂肪球表皮生长因子 8 在糖尿病性动脉损伤中的关键作用。
PLoS One. 2012;7(12):e52541. doi: 10.1371/journal.pone.0052541. Epub 2012 Dec 21.
6
Protective effects of dietary arginine supplementation against oxidative stress in weaned piglets.日粮添加精氨酸对断奶仔猪氧化应激的保护作用。
Br J Nutr. 2013 Jun 28;109(12):2253-60. doi: 10.1017/S0007114512004321. Epub 2012 Nov 26.
7
Physiological roles of mitochondrial reactive oxygen species.线粒体活性氧的生理作用。
Mol Cell. 2012 Oct 26;48(2):158-67. doi: 10.1016/j.molcel.2012.09.025.
8
The effects of oxidative stress on female reproduction: a review.氧化应激对女性生殖的影响：综述。
Reprod Biol Endocrinol. 2012 Jun 29;10:49. doi: 10.1186/1477-7827-10-49.
9
Insulin-like growth factor-1 induces the phosphorylation of PRAS40 via the PI3K/Akt signaling pathway in PC12 cells.胰岛素样生长因子-1 通过 PI3K/Akt 信号通路诱导 PC12 细胞中 PRAS40 的磷酸化。
Neurosci Lett. 2012 May 10;516(1):105-9. doi: 10.1016/j.neulet.2012.03.068. Epub 2012 Apr 2.
10
Follicular growth and atresia in mammalian ovaries: regulation by survival and death of granulosa cells.哺乳动物卵巢中的卵泡生长与闭锁：由颗粒细胞的存活和死亡调控
J Reprod Dev. 2012;58(1):44-50. doi: 10.1262/jrd.2011-012.