Thakur Pallavi, Chawla Raman, Narula Alka, Goel Rajeev, Arora Rajesh, Sharma Rakesh Kumar
Division of CBRN Defence, Institute of Nuclear Medicine and Allied Sciences, Delhi, India.
Division of CBRN Defence, Institute of Nuclear Medicine and Allied Sciences, Delhi, India.
Microb Pathog. 2016 Jun;95:133-141. doi: 10.1016/j.micpath.2016.04.005. Epub 2016 Apr 4.
Expression of a multitude of virulence factors by multi-drug resistant microbial strains, e.g., Carbapenem Resistant Escherichia coli (Family: Enterobacteriaceae; Class: Gammaproteobacteria), is responsible for resistance against beta-lactam antibiotics. Hemolysin production and induction of hemagglutination by bacterial surface receptors inflicts direct cytotoxicity by destroying host phagocytic and epithelial cells. We have previously reported that Berberis aristata, Camellia sinensis, Cyperus rotundus Holarrhena antidysenterica and Andrographis paniculata are promising herbal leads for targeting Carbapenem resistant Escherichia coli. These herbal leads were analyzed for their anti-hemolytic potential by employing spectrophotometric assay of hemoglobin liberation. Anti-hemagglutination potential of the extracts was assessed by employing qualitative assay of visible RBC aggregate formation. Camellia sinensis (PTRC-31911-A) exhibited anti-hemolytic potential of 73.97 ± 0.03%, followed by Holarrhena antidysenterica (PTRC-8111-A) i.e., 68.32 ± 0.05%, Berberis aristata (PTRC-2111-A) i.e., 60.26 ± 0.05% and Cyperus rotundus (PTRC-31811-A) i.e., 53.76 ± 0.03%. Comprehensive, visual analysis of hemagglutination inhibition revealed that only Berberis aristata (PTRC-2111-A) and Camellia sinensis (PTRC-31911-A) exhibited anti-hemagglutination activity. However, Andrographis paniculata (PTRC-11611-A) exhibited none of the inhibitory activities. Furthermore, the pair wise correlation analysis of the tested activities with quantitative phytochemical descriptors revealed that an increased content of alkaloid; flavonoids; polyphenols, and decreased content of saponins supported both the activities. Additionally, flow cytometry revealed that cell membrane structures of CRE were damaged by extracts of Berberis aristata (PTRC-2111-A) and Camellia sinensis (PTRC-31911-A) at their respective Minimum Inhibitory Concentrations, thereby confirming noteworthy antibacterial potential of both these extracts targeting bacterial membrane; hemolysin and bacterial hemagglutination.
多重耐药微生物菌株，例如耐碳青霉烯类大肠杆菌（科：肠杆菌科；纲：γ-变形菌纲）表达多种毒力因子，这导致了对β-内酰胺类抗生素的耐药性。细菌表面受体产生溶血素并诱导血细胞凝集，通过破坏宿主吞噬细胞和上皮细胞造成直接细胞毒性。我们之前报道过，刺檗、茶、香附、止泻木和穿心莲是针对耐碳青霉烯类大肠杆菌的有前景的草药先导物。通过血红蛋白释放的分光光度法测定，分析了这些草药先导物的抗溶血潜力。通过可见红细胞聚集体形成的定性测定评估提取物的抗血细胞凝集潜力。茶（PTRC - 31911 - A）表现出73.97±0.03%的抗溶血潜力，其次是止泻木（PTRC - 8111 - A），即68.32±0.05%，刺檗（PTRC - 2111 - A），即60.26±0.05%，以及香附（PTRC - 31811 - A），即53.76±0.03%。对血细胞凝集抑制的全面视觉分析表明，只有刺檗（PTRC - 2111 - A）和茶（PTRC - �1911 - A）表现出抗血细胞凝集活性。然而，穿心莲（PTRC - 11611 - A）没有表现出任何抑制活性。此外，将测试活性与定量植物化学描述符进行成对相关性分析发现，生物碱、黄酮类化合物、多酚含量增加以及皂苷含量降低支持了这两种活性。此外，流式细胞术显示，在各自的最低抑菌浓度下，刺檗（PTRC - 2111 - A）和茶（PTRC - 3\1911 - A）的提取物会破坏耐碳青霉烯类大肠杆菌的细胞膜结构，从而证实了这两种提取物针对细菌膜、溶血素和细菌血细胞凝集具有显著的抗菌潜力。
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