Division of CBRN Defence, Institute of Nuclear Medicine and Allied Sciences, New Delhi 110054, India.
Division of CBRN Defence, Institute of Nuclear Medicine and Allied Sciences, New Delhi 110054, India.
J Glob Antimicrob Resist. 2017 Jun;9:21-29. doi: 10.1016/j.jgar.2016.12.018. Epub 2017 Mar 18.
Berberis aristata is known to contain a variety of phenolic compounds contributing to its holistic capability of mitigating bacterial multidrug resistance.
B. aristata stem bark extract was prepared and was characterised using liquid chromatography-mass spectrometry (LC-MS). The antimicrobial efficacy of the extract against carbapenem-resistant Escherichia coli was assessed in vivo in an animal model using Sprague Dawley rats. Microbial counts in blood and urine, physical health status, haematological and biochemical analysis of blood, and histopathology of the kidney were assessed as the study endpoints.
An aquo-alcoholic extract of B. aristata (PTRC-2111-A) was found to effectively manage peritonitis induced by carbapenem-resistant E. coli in a rat model at a single post-exposure prophylactic dose of 0.5mg/kg body weight (BW). The extract was also found to show a no observed adverse effect level (NOAEL) up to a dose of 2000mg/kg BW. Physical, immunological, haematological, biochemical and histopathological aberrations were found to be restored to normal in the herbal-treated group at a dose of 0.5mg/kg BW.
The antimicrobial and hepatorenal protective ability of PTRC-2111-A could be attributed to the presence of isoquinoline alkaloids.
小檗属植物含有多种酚类化合物，这使其具有整体减轻细菌多重耐药性的能力。
从小檗属植物茎皮中提取并制备提取物，并使用液相色谱-质谱联用（LC-MS）进行鉴定。在使用 Sprague Dawley 大鼠的动物模型中评估该提取物对耐碳青霉烯类大肠埃希菌的抗菌功效。将血液和尿液中的微生物计数、身体健康状况、血液的血液学和生化学分析以及肾脏的组织病理学作为研究终点进行评估。
发现小檗属植物的水醇提取物（PTRC-2111-A）在单次暴露后预防剂量为 0.5mg/kg 体重（BW）时，可有效治疗耐碳青霉烯类大肠埃希菌引起的腹膜炎。还发现该提取物在 2000mg/kg BW 剂量下没有观察到不良反应水平（NOAEL）。在 0.5mg/kg BW 剂量下，草药治疗组的身体、免疫、血液、生化和组织病理学异常均恢复正常。
PTRC-2111-A 的抗菌和肝肾功能保护能力可能归因于其存在异喹啉生物碱。
