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采用 UPLC-MS 法评价两种李属果实(Spondias spp.)的抗氧化能力、对人红细胞的保护作用及酚类化合物的鉴定。

Evaluation of Antioxidant Capacity, Protective Effect on Human Erythrocytes and Phenolic Compound Identification in Two Varieties of Plum Fruit (Spondias spp.) by UPLC-MS.

机构信息

Departamento de Biotecnología y Ciencias Alimentarias, Instituto Tecnológico de Sonora, 85000 Ciudad Obregón, Sonora, México.

Centro de Investigación en Alimentación y Desarrollo. Av. Río Conchos S/N Parque Industrial, 31570 Cuauhtémoc, Chihuahua, México.

出版信息

Molecules. 2018 Dec 4;23(12):3200. doi: 10.3390/molecules23123200.

DOI:10.3390/molecules23123200
PMID:30518166
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6321542/
Abstract

Plum edible part was used to obtained extracts by during a 4 h maceration process using three different solvents (ethanol, methanol and water) for the determination of total phenols and flavonoids, antioxidant capacity by (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hemolysis inhibition in human blood assays. Subsequently, phenolic compounds were identified using ultra-performance liquid chromatography (UPLC-MS). The results indicated that the ethanolic extract of plum fruit being a good source of phenolic (12⁻18 mg GAE/g FW) and flavonoids (2.3⁻2.5 mg QE/g FW) content in both varieties of plum. Also, the fruits proved a good source of antioxidants as measured by DPPH and ABTS; likewise, plum aqueous extracts showed the highest protective effect on human erythrocytes with 74.34 and 64.62% for yellow and red plum, respectively. A total of 23 bioactive compounds were identified by UPLC-MS, including gallic acid, rutin, resorcinol, chlorogenic acid, catechin, and ellagic acid, and the antioxidant capacity can be attributed to these species. The edible part of plum contains compounds of biological interest, suggesting that this fruit has antioxidant potential that can be exploited for various technologies.

摘要

李子的可食用部分在 4 小时的浸泡过程中分别使用三种不同溶剂(乙醇、甲醇和水)来提取,以测定总酚和类黄酮、(2,2'- 连氮-双(3-乙基苯并噻唑啉-6-磺酸)二铵盐(ABTS)、2,2-二苯基-1-苦基肼(DPPH)的抗氧化能力以及人血抑制溶血的能力。随后,使用超高效液相色谱(UPLC-MS)对酚类化合物进行了鉴定。结果表明,李子果实的乙醇提取物是酚类化合物(12⁻18 mg GAE/g FW)和类黄酮(2.3⁻2.5 mg QE/g FW)的良好来源,这两种李子品种都有此特性。此外,这些水果被证明是 DPPH 和 ABTS 测定的良好抗氧化剂来源;同样,李子水提取物对人红细胞表现出最高的保护作用,黄色和红色李子的分别为 74.34%和 64.62%。通过 UPLC-MS 共鉴定出 23 种生物活性化合物,包括没食子酸、芦丁、间苯二酚、绿原酸、儿茶素和鞣花酸,抗氧化能力归因于这些物质。李子的可食用部分含有具有生物活性的化合物,表明这种水果具有抗氧化潜力,可以应用于各种技术。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0eda/6321542/abd3cc348abc/molecules-23-03200-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0eda/6321542/1e5e2ad51d54/molecules-23-03200-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0eda/6321542/125389033c7f/molecules-23-03200-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0eda/6321542/56da050fdaaf/molecules-23-03200-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0eda/6321542/abd3cc348abc/molecules-23-03200-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0eda/6321542/1e5e2ad51d54/molecules-23-03200-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0eda/6321542/125389033c7f/molecules-23-03200-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0eda/6321542/56da050fdaaf/molecules-23-03200-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0eda/6321542/abd3cc348abc/molecules-23-03200-g004.jpg

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