Thakur Pallavi, Chawla Raman, Narula Alka, Goel Rajeev, Arora Rajesh, Sharma Rakesh Kumar
J Complement Integr Med. 2016 Sep 1;13(3):229-237. doi: 10.1515/jcim-2015-0066.
Berberis aristata is known to contain a variety of phenolic compounds, flavonoids such as quercetin attributing towards its holistic capability of mitigating multidrug resistance.
B. aristata stem bark extract was prepared and characterized using phytochemical and bioactivity-based fingerprinting. Anti-oxidant and anti-lipid peroxidation profiling was also done in conjunction with in vitro anti-microbial efficacy testing against the test microorganism i. e., New Delhi Metallo-β-lactamase-1 (NDM-1) Escherichia coli.
Aquo-alcoholic (1:1) extract of B. aristata (PTRC-2111-A), containing 3.0±0.02 µg of QUERCETIN/mg of dried extract, exhibited [flavonoid/polyphenol: F/P (quercetin %) ~ 0.16(0.06 %)]. The bioactivity fingerprint profile of PTRC-2111-A included IC50 ratio [DPPH/NOS]=0.064 as functional standardized value having IC50 (DPPH Scavenging)=16±0.5 µg/mL and IC50 (Nitric Oxide Scavenging)=250±0.5 µg/mL respectively. The reducing ability and anti-lipid peroxidation equivalent (extract: standard) of PTRC-2111-A with respect to standard was estimated to be 3.44 (ascorbic acid) and 0.78 (quercetin) respectively. In vitro anti-microbial activity evaluated against sts-09 multidrug-resistant strain of carbapenem-resistant E. coli was found to be 25 µg/mL.
B. aristata was found to contain a number of phytoconstituents, which acts in a synergistic manner to provide significant bactericidal potential against carbapenem-resistant E. coli.
已知刺檗含有多种酚类化合物、黄酮类化合物，如槲皮素，这归因于其减轻多药耐药性的整体能力。
制备刺檗茎皮提取物，并使用基于植物化学和生物活性的指纹图谱进行表征。还进行了抗氧化和抗脂质过氧化分析，并结合对测试微生物即新德里金属β-内酰胺酶-1（NDM-1）大肠杆菌的体外抗菌功效测试。
刺檗（PTRC-2111-A）的水醇（1:1）提取物，每毫克干提取物含3.0±0.02微克槲皮素，表现出[黄酮类化合物/多酚：F/P（槲皮素%）~0.16（0.06%）]。PTRC-2111-A的生物活性指纹图谱包括IC50比值[DPPH/NOS]=0.064作为功能标准化值，其IC50（DPPH清除率）=16±0.5微克/毫升，IC50（一氧化氮清除率）=250±0.5微克/毫升。PTRC-2111-A相对于标准品的还原能力和抗脂质过氧化当量（提取物：标准品）估计分别为3.44（抗坏血酸）和0.78（槲皮素）。对碳青霉烯耐药大肠杆菌的sts-09多药耐药菌株进行的体外抗菌活性测定为25微克/毫升。
发现刺檗含有多种植物成分，它们以协同方式发挥作用，对碳青霉烯耐药大肠杆菌具有显著的杀菌潜力。
