miR-375通过靶向BMPR2对猪前脂肪细胞分化起负调控作用。

miR-375 negatively regulates porcine preadipocyte differentiation by targeting BMPR2.

作者信息

Liu Siyuan, Sun Guangjie, Yuan Bao, Zhang Lianjiang, Gao Yan, Jiang Hao, Dai Lisheng, Zhang Jiabao

机构信息

College of Animal Sciences, Jilin University, Changchun, China.

College of Animal Science and Technology, Jilin Agricultural Science and Technology College, China.

出版信息

FEBS Lett. 2016 May;590(10):1417-27. doi: 10.1002/1873-3468.12169. Epub 2016 May 12.

DOI:10.1002/1873-3468.12169

PMID:27059117

Abstract

The differentiation of preadipocytes into adipose tissues is tightly regulated by various factors including miRNAs and cytokines. In this study, taking advantage of isolated porcine primary preadipocytes, we showed that ectopic expression of miR-375 could change preadipocyte differentiation. In addition, bone morphogenetic protein receptor 2 (BMPR2) was identified as a direct target of miR-375. Silencing BMPR2 had the same inhibition effects as overexpressing miR-375 on the preadipocyte differentiation. Together, we demonstrated that miR-375 is a negative regulator of adipogenic differentiation using porcine primary preadipocytes. These results clarified the role of miR-375 in ex vivo adipogenic differentiation.

摘要

前脂肪细胞向脂肪组织的分化受到包括微小RNA（miRNAs）和细胞因子在内的多种因素的严格调控。在本研究中，利用分离的猪原代前脂肪细胞，我们发现miR-375的异位表达可改变前脂肪细胞的分化。此外，骨形态发生蛋白受体2（BMPR2）被鉴定为miR-375的直接靶点。沉默BMPR2对前脂肪细胞分化的抑制作用与过表达miR-375相同。我们共同证明，利用猪原代前脂肪细胞，miR-375是脂肪生成分化的负调节因子。这些结果阐明了miR-375在体外脂肪生成分化中的作用。