Shi Tao, Yan Xiaoru, Qiao Liying, Li Baojun, Cheng Lifen, Pan Yangyang, Jing Jiongjie, Cao Ningxian, Liu Wenzhong
College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, China.
Division of Animal and Poultry Breeding, Department of Agriculture of Shanxi Province, Taiyuan, China.
Anim Sci J. 2018 Jun;89(6):858-867. doi: 10.1111/asj.12995. Epub 2018 Mar 25.
The differentiation of preadipocytes into adipose tissues is tightly regulated by various factors including microRNAs and cytokines. This article aims to study the effect of miR-330-5p on expression of BCAT2 in ovine preadipocytes. Ovine preadipocytes were isolated, and we found that the miR-330-5p expression decreased gradually during the early differentiation of ovine preadipocytes, while BCAT2 expression increased. BCAT2 was identified as a direct target of miR-330-5p, ectopic expression of miR-330-5p could change the expression of both BCAT2 mRNA and protein. Silencing BCAT2 had the same inhibition effects as overexpressing miR-330-5p on the preadipocyte differentiation, but overexpressing BCAT2 had the converse effects. Taken together, we demonstrated that miR-330-5p is a negative regulator of differentiation by targeting BCAT2, and clarified the role of BCAT2 and miR-330-5p during preadipocyte differentiation.
前体脂肪细胞向脂肪组织的分化受到包括微小RNA和细胞因子在内的多种因素的严格调控。本文旨在研究miR-330-5p对绵羊前体脂肪细胞中BCAT2表达的影响。分离出绵羊前体脂肪细胞，我们发现miR-330-5p在绵羊前体脂肪细胞早期分化过程中表达逐渐降低，而BCAT2表达升高。BCAT2被鉴定为miR-330-5p的直接靶标，miR-330-5p的异位表达可改变BCAT2 mRNA和蛋白的表达。沉默BCAT2对前体脂肪细胞分化的抑制作用与过表达miR-330-5p相同，但过表达BCAT2则产生相反的效果。综上所述，我们证明miR-330-5p通过靶向BCAT2是分化的负调节因子，并阐明了BCAT2和miR-330-5p在前体脂肪细胞分化过程中的作用。
