Galler Kerstin, Requardt Robert Pascal, Glaser Uwe, Markwart Robby, Bocklitz Thomas, Bauer Michael, Popp Jürgen, Neugebauer Ute
Leibniz Institute of Photonic Technology, Jena, Germany.
Center for Sepsis Control and Care, Jena University Hospital, Germany.
Sci Rep. 2016 Apr 11;6:24155. doi: 10.1038/srep24155.
Hepatic stellate cells (HSCs) are retinoid storing cells in the liver: The retinoid content of those cells changes depending on nutrition and stress level. There are also differences with regard to a HSC's anatomical position in the liver. Up to now, retinoid levels were only accessible from bulk measurements of tissue homogenates or cell extracts. Unfortunately, they do not account for the intercellular variability. Herein, Raman spectroscopy relying on excitation by the minimally destructive wavelength 785 nm is introduced for the assessment of the retinoid state of single HSCs in freshly isolated, unprocessed murine liver lobes. A quantitative estimation of the cellular retinoid content is derived. Implications of the retinoid content on hepatic health state are reported. The Raman-based results are integrated with histological assessments of the tissue samples. This spectroscopic approach enables single cell analysis regarding an important cellular feature in unharmed tissue.
肝星状细胞（HSCs）是肝脏中储存视黄醇的细胞：这些细胞中的视黄醇含量会根据营养和应激水平而变化。肝星状细胞在肝脏中的解剖位置也存在差异。到目前为止，视黄醇水平只能通过组织匀浆或细胞提取物的大量测量来获取。不幸的是，这些测量无法反映细胞间的变异性。本文介绍了一种基于拉曼光谱的方法，该方法利用785 nm的最小破坏性激发波长来评估新鲜分离、未处理的小鼠肝叶中单个肝星状细胞的视黄醇状态。由此得出了细胞视黄醇含量的定量估计值，并报告了视黄醇含量对肝脏健康状态的影响。基于拉曼光谱的结果与组织样本的组织学评估相结合。这种光谱方法能够对未受损组织中的重要细胞特征进行单细胞分析。
