Patterson G W
Department of Botany, University of Maryland, College Park, Maryland 20742.
J Phycol. 1967 Mar;3(1):22-3. doi: 10.1111/j.1529-8817.1967.tb04623.x.
Cultures of Chlorella vulgaris were grown aulo-trophically under fluorescent light and heterotrophically on glucose and inorganic salts. Hydrocarbons were extracted and analyzed by gas-liquid chromatography, molecular sieve separations, and silicic acid-AgNO3 chromatography. Chlorella vulgaris grown under both culture conditions contained a series of saturated n-paraffins ranging from 17 to 36 carbon atoms in length. This is in contrast to reports in the early literature which indicated that the hydrocarbon fraction of algae was composed of only 1 or 2 specific hydrocarbons. Only under heterotrophic conditions, however, did C. vulgaris produce 1-penta-cosene and 1-heptacosene as the primary components of the hydrocarbon mixture. Other Chlorella species were examined, but only C. vulgaris produced significant quantities of these compounds.
小球藻在荧光灯下自养生长，并在葡萄糖和无机盐上异养生长。通过气液色谱法、分子筛分离法和硅酸 - 硝酸银色谱法提取并分析碳氢化合物。在两种培养条件下生长的小球藻都含有一系列长度从17到36个碳原子的饱和正构烷烃。这与早期文献报道不同，早期文献表明藻类的碳氢化合物部分仅由1种或2种特定的碳氢化合物组成。然而，只有在异养条件下，小球藻才产生1 - 二十五碳烯和1 - 二十七碳烯作为碳氢化合物混合物的主要成分。对其他小球藻物种进行了检测，但只有普通小球藻产生了大量这些化合物。
