Shalaby Hatem A, El Namaky Amira H, Kamel Reem O A
Department of Parasitology and Animal Diseases, National Research Center, Giza, Egypt.
Department of Zoology, Girls College for Art, Science and Education, Ain Shams University, Cairo, Egypt.
J Parasit Dis. 2016 Mar;40(1):145-51. doi: 10.1007/s12639-014-0466-y. Epub 2014 Apr 26.
Emergence of drug-resistant Fasciola strains has drawn the attention of many authors to alternative drugs. The purpose of this study is to explore the in vitro effect of the antimalarial mefloquine against adult Fasciola gigantica. Light and scanning electron microscopic observations could be used to determine the target of the drug following 6 and 12 h of incubation in medium containing mefloquine at three different concentrations 10, 20 and 30 μg/mL, as morphological changes could be observed. These changes occurred in definite sequences in response to mefloquine, and were consisted of swelling, vacuolization that was later disrupted, leading to desquamation of the tegument, resulting in exposure and disruption of basal lamina and the dislodging of spines. It is concluded that mefloquine presented itself as a drug that might become important in trematode chemotherapy, with the tegument being an important drug target.
耐药性肝片吸虫菌株的出现引起了许多作者对替代药物的关注。本研究的目的是探讨抗疟药甲氟喹对成年巨片形吸虫的体外作用。在含有三种不同浓度（10、20和30μg/mL）甲氟喹的培养基中孵育6小时和12小时后，可通过光学显微镜和扫描电子显微镜观察来确定药物的作用靶点，因为可以观察到形态变化。这些变化是由甲氟喹引起的，按一定顺序发生，包括肿胀、空泡化，随后空泡破裂，导致体表脱屑，从而使基膜暴露和破裂以及棘突脱落。得出的结论是，甲氟喹可能成为吸虫化疗中的一种重要药物，体表是一个重要的药物作用靶点。
